PhenoImager Assays, Kits and Reagents
Shifting the paradigm from visual IHC to quantitative IF with Opal
We’ve rebranded some of our products, learn more ›
CODEX is now PhenoCycler
Phenoptics is now Phenolmager
Image Multiple Tissue Biomarkers in Context
Akoya’s Opal™ Multiplex Detection Kits make multiplex results accessible to anyone who works with standard immunohistochemistry. With Opal technology, you can select antibodies for simultaneous IHC detection based on performance rather than species. Opal kits are optimized for reliable Multispectral Imaging (MSI) for simultaneous measurement of three to eight IHC targets, plus a nuclear stain.
Opal enables you to:
- Accomplish better phenotypic scoring and quantification by using signal amplification
- Reduce overall sample and reagent consumption with increased plexing
- Achieve excellent resolution with low background
- Reach up to 4 logs dynamic range compared to 1 log using chromogenic assay detection techniques
Opal Panels, Detection Kits & Reagents
Opal Predesigned Panels for Automated PhenoImager Workflows
Opal Antibody Panels provide pre-optimized, ready-to-use primary antibodies and detection reagents designed for use with automated staining and PhenoImager® Fusion and PhenoImager® HT (formerly Vectra Polaris) instruments and offer a validated end-to-end solution for unparalleled quantitative data in translational immuno-oncology research. MOTiF™ PD1/PD-L1 Panel: Auto Lung Cancer Kit and MOTiF PD-1/PD-L1 Panel: Auto Melanoma Kit have been renamed as shown below.
Innovative Rapid Whole Slide Panels
Key Benefits:
- Easy-to-Use – Fully validated plug-and-play multiplex immunofluorescence staining protocol.
- Flexible – Adjustable signal intensity for your sample’s unique biomarker expression levels.
- Powerful – Unlock the full impact of whole slide multispectral workflows with Akoya’s kits when used with the PhenoImager Fusion and PhenoImager HT (formerly Vectra Polaris) and inForm analysis software.
Opal Multiplex Detection Kits
Opal for multiplex immunofluorescence in formalin-fixed, paraffin-embedded (FFPE) tissue can be performed manually or via automation. The Opal detection system enables the development of multiplexed assays with balanced, quantitative signal for rare and abundant targets, imaged in a single, final scan with the Akoya portfolio of multispectral imagers, including the PhenoImager Fusion and PhenoImager HT. The Opal Multiplex Detection Kits have been renamed to reflect the level of multiplexing instead of numbers of colors. Automation has become the default and “Manual” compatibility is indicated where applicable.
Automated Opal Multiplex Detection Kits
These Opal detection kits allow user to perform multiplex staining using an automated staining platform. Automation provides the flexibility to support the dynamic demands of translational research
Catalog #
Name
Opal 6-Plex Detection Kit - for Whole Slide Imaging
Opal 6-Plex Detection Kit
Opal 3-Plex Detection Kit
Opal 3-Plex Anti-Rb Detection Kit
Manual Opal Multiplex Predesigned Panels and Detection Kits
These Opal kits offer users a manual method that is similar to standard immunohistochemistry (IHC) making them accessible to many laboratories where standard IHC method development is performed.
Catalog #
Name
Opal 6-Plex Manual Detection Kit - for Whole Slide Imaging
Opal 6-Plex Manual Detection Kit
Opal 3-Plex Manual Detection Kit
Solid Tumor Immunology 6-Plex Panel
Tumor Infiltrating Lymphocyte 6-Plex Panel
Immunology Discovery Panel
Lymphocyte 3-Plex Panel
Opal 3-Plex Anti-Rb Manual Detection Kit
Opal Fluorophore Reagent Packs
Standalone Opal Fluorophore reagent packs allow you to pick and choose your panel’s biomarker quantity and design.
Catalog #
Name
Opal 480 Reagent Pack
Opal 520 Reagent Pack
Opal 540 Reagent Pack
Opal 570 Reagent Pack
Opal 620 Reagent Pack
Opal 650 Reagent Pack
Opal 690 Reagent Pack
Opal 780 Reagent Pack
10X Spectral DAPI
Opal Reagents: Essentials for Staining
These ready-to-use reagents, from diluents to secondary antibodies and blocking buffers, have been optimized specifically for Opal™ multiplex fluorescent immunohistochemistry protocols
Catalog #
Name
1X Plus Manual Amplification Diluent
1X Plus Automation Amplification Diluent
1X Antibody Diluent / Block
10X AR9 Buffer
10X AR6 Buffer
1X Opal Anti-Ms + Rb HRP
Opal Anti-Rb HRP Kit
TSA Reagents
Tyramide signal amplification (TSA® staining) typically provides 2-3 logs of sensitivity enhancement over standard fluorescent and hapten based detection methods. TSA staining increases sensitivity while maintaining resolution and dynamic range while allowing reduced consumption of primary antibodies.
Protocol optimization for TSA detection is critical for success with the technique, whether used as part of the PhenoImager multiplex imaging instruments or other detection system.
TSA Reagents for IHC, IF, or ISH
TSA® and TSA® Plus fluorophore and hapten labeled detection kits, for detection and amplification of signals in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols.
Catalog #
Name
Size
Related Publications
Clinical workflow for quantifying PD-L1 in non-small cell lung cancer (NSCLC)
Professor Manuel Salto-Tellez, MD
Chair of Molecular Pathology, Queen’s University Belfast
Chair of Molecular Pathology, Queen’s University Belfast
Researchers at Queen’s University demonstrate that digital image analysis (DIA) of mIF using Opal 7-Color Automation IHC Kit is highly comparable to DIA on DAB IHC slides
Multi-institutional TSA-amplified Multiplexed Immunofluorescence Reproducibility Evaluation (MITRE) Study
Janis Taube, MD, MSc, et al.,
The Johns Hopkins Hospital, Akoya Biosciences, Providence Cancer Institute, MD Anderson Cancer Center, Bristol Myers Squibb, Yale University School of Medicine
The Johns Hopkins Hospital, Akoya Biosciences, Providence Cancer Institute, MD Anderson Cancer Center, Bristol Myers Squibb, Yale University School of Medicine
A collaborative study across 6 institutions demonstrated that an Opal based six-plex mIF panel used for the characterization of the PD-1/PD-L1 axis across multiple sites was highly sensitive with high concordance and reproducibility observed across the sites.
Characterizing the TIME with TSA-Based Multiplex Immunofluorescence
Alexander D. Borowsky,
MDUniversity of California Davis
MDUniversity of California Davis
This UC Davis led study developed mIF protocols incorporating Opal tyramide signal amplification (TSA) for immune profiling the tumor microenvironment
Related Resources
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Case Studies
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