PhenoImager Assays, Kits and Reagents
Shifting the paradigm from visual IHC to quantitative IF with Opal
We’ve rebranded some of our products, learn more ›
CODEX is now PhenoCycler
Phenoptics is now Phenolmager
Introducing PhenoCode Signature Panels

Whether you’re developing predictive biomarkers, understanding response to immunotherapy, developing the next generation of immunotherapies, or validating your biomarker studies, PhenoCode™ Signature Panels provide the flexibility to keep pace with the dynamic combination therapy landscape.
PhenoCode Signature Panels
PhenoCode Signature panels blend the sensitivity of the Opal-TSA detection system with the flexibility and easy assay development features of Akoya's propiertary universal protein barcoding chemistry. These panels enable comprehensive analysis of key aspects of the tumor microenvironment involved in immune response, providing a rapid and novel solution for the development of a spatial signature.
PhenoCode Signature Panels
Featuring a flexible design component, PhenoCode Signature panels allow for the easy integration of a novel checkpoint or immune cell marker into a 5-plex base panel. Panels include primary antibodies, Opal signal amplification dyes, and reagents to accommodate 1 additional biomarker of interest to answer your question of interest.
Antibodies for PhenoCode Signature
Choose from the list below to add your "+1" antibody into a PhenoCode Signature Panel.
Kits and Reagents for PhenoCode Signature
Detection Reagents
Opal Reagent Packs
Opals Reagent Packs include an Opal fluorophore dye packaged with DMSO for dye reconstitution. The Opal system provides TSA-based signal amplification to detect co-localized biomarkers at sub-cellular sensitivity.
Kits for Multiplexing
Opal Antibody Panels provide pre-optimized, ready-to-use primary antibodies and detection reagents designed for use with automated staining and PhenoImager™ Fusion and PhenoImager™ HT (formerly Vectra Polaris) instruments and offer a validated end-to-end solution for unparalleled quantitative data in translational immuno-oncology research.
The Opal Multiplex Detection Kits have been renamed to reflect the level of multiplexing instead of numbers of colors. Automation has become the default and “Manual” compatibility is indicated where applicable.
Staining Essentials
These ready-to-use reagents, from diluents to secondary antibodies and blocking buffers, have been optimized specifically for Opal™ multiplex fluorescent immunohistochemistry protocols.
TSA Reagents
Tyramide signal amplification (TSA™ staining) typically provides 2-3 logs of sensitivity enhancement over standard fluorescent and hapten based detection methods. TSA staining increases sensitivity while maintaining resolution and dynamic range while allowing reduced consumption of primary antibodies.
Related Publications
Clinical workflow for quantifying PD-L1 in non-small cell lung cancer (NSCLC)
Chair of Molecular Pathology, Queen’s University Belfast
Multi-institutional TSA-amplified Multiplexed Immunofluorescence Reproducibility Evaluation (MITRE) Study
The Johns Hopkins Hospital, Akoya Biosciences, Providence Cancer Institute, MD Anderson Cancer Center, Bristol Myers Squibb, Yale University School of Medicine
Characterizing the TIME with TSA-Based Multiplex Immunofluorescence
MDUniversity of California Davis