PhenoImager Assays, Kits and Reagents

Shifting the paradigm from visual IHC to quantitative IF with Opal

We’ve rebranded some of our products, learn more ›

CODEX is now PhenoCycler
Phenoptics is now Phenolmager

Introducing PhenoCode Signature Panels

Whether you’re developing predictive biomarkers, understanding response to immunotherapy, developing the next generation of immunotherapies, or validating your biomarker studies, PhenoCode™ Signature Panels provide the flexibility to keep pace with the dynamic combination therapy landscape.

PhenoCode Signature Panels

PhenoCode Signature panels blend the sensitivity of the Opal-TSA detection system with the flexibility and easy assay development features of Akoya's propiertary universal protein barcoding chemistry. These panels enable comprehensive analysis of key aspects of the tumor microenvironment involved in immune response, providing a rapid and novel solution for the development of a spatial signature.

PhenoCode Signature Panels

Featuring a flexible design component, PhenoCode Signature panels allow for the easy integration of a novel checkpoint or immune cell marker into a 5-plex base panel. Panels include primary antibodies, Opal signal amplification dyes, and reagents to accommodate 1 additional biomarker of interest to answer your question of interest.

Catalog #
Name
PhenoCode Signature Immuno-Contexture Human Protein Panel (CD8, CD68, PD-L1, FoxP3, PanCK)
PhenoCode Signature Immune Profile Human Protein Panel (CD8, CD3, CD68, CD20, PanCK)
PhenoCode Signature Activated TIL Status Human Protein Panel (CD8, CD3, Ki-67, Granzyme B, PanCK)

Antibodies for PhenoCode Signature

Choose from the list below to add your "+1" antibody into a PhenoCode Signature Panel.

Catalog #
Name
Anti-Hu Pan-Cytokeratin(AKYP0053)-BX066 for PhenoCode Signature
Anti-Hu CD8(AKYP0028)-BX026 for PhenoCode Signature
Anti-Hu CD4(AKYP0048)-BX003 for PhenoCode Signature
Anti-Hu CD3e(AKYP0125)-BX080 for PhenoCode Signature
Anti-Hu CD68(AKYP0050)-BX015 for PhenoCode Signature
Anti-Hu Ki67(AKYP0126)-BX047 for PhenoCode Signature
Anti-Hu FOXP3(AKYP0102)-BX031 for PhenoCode Signature
Anti-Hu CD20(AKYP0049)-BX064 for PhenoCode Signature
Anti-Hu Granzyme B(AKYP0086)-BX041 for PhenoCode Signature
Anti-Hu PD-1(AKYP0127)-BX046 for PhenoCode Signature
Anti-Hu PD-L1(AKYP0103)-BX067 for PhenoCode Signature

Kits and Reagents for PhenoCode Signature

Catalog #
Name
Antibody Conjugation Kit for PhenoCode Signature
Staining Kit for PhenoCode Signature
PhenoCode Signature Diluent

Detection Reagents

Opal Reagent Packs

Opals Reagent Packs include an Opal fluorophore dye packaged with DMSO for dye reconstitution. The Opal system provides TSA-based signal amplification to detect co-localized biomarkers at sub-cellular sensitivity.

Catalog #
Name
Opal 480 Reagent Pack
Opal 520 Reagent Pack
Opal 540 Reagent Pack
Opal 570 Reagent Pack
Opal 620 Reagent Pack
Opal 650 Reagent Pack
Opal 690 Reagent Pack
Opal 780 Reagent Pack
10X Spectral DAPI

Opal Antibody Panels provide pre-optimized, ready-to-use primary antibodies and detection reagents designed for use with automated staining and PhenoImager™ Fusion and PhenoImager™ HT (formerly Vectra Polaris) instruments and offer a validated end-to-end solution for unparalleled quantitative data in translational immuno-oncology research.

The Opal Multiplex Detection Kits have been renamed to reflect the level of multiplexing instead of numbers of colors. Automation has become the default and “Manual” compatibility is indicated where applicable.

Catalog #
Name
Opal 6-Plex Detection Kit - for Whole Slide Imaging (for use with an autostainer)
Opal 6-Plex Manual Detection Kit - for Whole Slide Imaging
Opal 6-Plex Detection Kit (for use with an autostainer)
Opal 6-Plex Manual Detection Kit
Opal 3-Plex Detection Kit (for use with an autostainer)
Opal 3-Plex Manual Detection Kit
Opal 3-Plex Anti-Rb Detection Kit (for use with an autostainer)
Opal 3-Plex anti-Rabbit Manual Kit
Lung Cancer 6-Plex Panel
Melanoma 6-Plex Panel
Solid Tumor Immunology 6-Plex Panel
Tumor Infiltrating Lymphocyte 6-Plex Panel
Immunology Discovery Panel Kit
Lymphocyte 3-Plex Panel

These ready-to-use reagents, from diluents to secondary antibodies and blocking buffers, have been optimized specifically for Opal™ multiplex fluorescent immunohistochemistry protocols.

Catalog #
Name
Size
10X AR6 Buffer
50-150 slides
10X AR9 Buffer
50-150 slides
1X Antibody Diluent / Block
50-150 slides
1X Plus Automation Amplification Diluent
50-150 slides
1X Plus Manual Amplification Diluent
50-150 slides
1X Opal Anti-Ms + Rb HRP
50-150 slides
Opal Anti-Rb HRP Kit
50-150 slides

Tyramide signal amplification (TSA™ staining) typically provides 2-3 logs of sensitivity enhancement over standard fluorescent and hapten based detection methods. TSA staining increases sensitivity while maintaining resolution and dynamic range while allowing reduced consumption of primary antibodies.

Catalog #
Name
Size
TSA Biotin
50-150 slides
TSA Plus DNP (HRP)
50-150 slides
TSA Plus DIG
50-150 slides
TSA Coumarin System
100–300 slides
TSA Cyanine 3
50-150 slides
TSA Plus Cyanine 3.5
50-150 slides
TSA Cyanine 5
50-150 slides
TSA Plus Cyanine 5.5
50-150 slides
TSA Fluorescein
50-150 slides
TSA TMR
50-150 slides

Related Publications

Clinical workflow for quantifying PD-L1 in non-small cell lung cancer (NSCLC)
Professor Manuel Salto-Tellez, MD
Chair of Molecular Pathology, Queen’s University Belfast
Researchers at Queen’s University demonstrate that digital image analysis (DIA) of mIF using Opal 7-Color Automation IHC Kit is highly comparable to DIA on DAB IHC slides
Multi-institutional TSA-amplified Multiplexed Immunofluorescence Reproducibility Evaluation (MITRE) Study
Janis Taube, MD, MSc, et al.,
The Johns Hopkins Hospital, Akoya Biosciences, Providence Cancer Institute, MD Anderson Cancer Center, Bristol Myers Squibb, Yale University School of Medicine
A collaborative study across 6 institutions demonstrated that an Opal based six-plex mIF panel used for the characterization of the PD-1/PD-L1 axis across multiple sites was highly sensitive with high concordance and reproducibility observed across the sites.
Characterizing the TIME with TSA-Based Multiplex Immunofluorescence
Alexander D. Borowsky,
MDUniversity of California Davis
This UC Davis led study developed mIF protocols incorporating Opal tyramide signal amplification (TSA) for immune profiling the tumor microenvironment

Related Resources