The PhenoCycler Assay

Optimized with Users in Mind

We’ve rebranded some of our products, learn more ›

CODEX is now PhenoCycler
Phenoptics is now Phenolmager

PhenoCycler Assays – Reagents and Antibodies

The PhenoCycler™ System (formerly CODEX®) overcomes challenges of traditional multiplexed immunofluorescence, by targeting subsets of the antibody panel through iterative imaging cycles, thus revealing 100+ biomarkers in a single tissue section.

We offer a complete solution that includes antibodies and reagents that have been optimized and validated for the PhenoCycler System. Validated and inventoried antibodies are available for different tissue types: Mouse Fresh Frozen, Human Fresh Frozen and Human FFPE. Users have the flexibility to create panels comprised of commercially available, Akoya-validated antibodies or clones labeled with PhenoCycler Barcodes using Akoya’s custom conjugation kit.

Akoya-Kit-Transparent-Backg

Kits & Reagents

Buffers and reagents for tissue staining, custom conjugations and performing PhenoCycler experiments
CODEX-Antibodies

PhenoCycler Antibodies

PhenoCycler conjugated antibodies for building antibody panels
CODEX-Barcodes

PhenoCycler Barcodes

Modified oligonucleotides for easy conjugation of custom PhenoCycler Antibodies
CODEX-Reporters

PhenoCycler Reporters

Fluorophore-conjugated oligonucleotides for visualization of PhenoCycler Antibodies

Kits & Reagents

The PhenoCycler System requires specially formulated reagents and buffers for tissue staining with PhenoCycler Antibodies and running iterative imaging cycles. For each imaging cycle, three PhenoCycler Reporters, each with a spectrally-distinct dye, are applied to the stained tissue to visualize the corresponding PhenoCycler Antibody. The PhenoCycler instrument applies gentle wash steps to remove the PhenoCycler Reporters without any chemical modifications, followed by applying the next set of Reporters to visualize the next set of PhenoCycler Antibodies.

Catalog #
Product
Size
10X PhenoCycler Buffer
10 reactions
PhenoCycler Assay Reagent
10 reactions
Nuclear Stain
10 reactions
PhenoCycler Staining Kit
10 reactions
PhenoCycler Conjugation Kit 10 reactions
10 reactions
PhenoCycler Gaskets v2
10 gaskets
PhenoCycler Storage Buffer
120 ml
Coverslips
138
96 well plates
10
96 well plate seals
10
PhenoCycler Coverslip Storage Box
1 box

PhenoCycler Antibodies

PhenoCycler Antibodies are provided individually and can be mixed and matched to design panels for the simultaneous analysis of up to 100+ biomarkers. Akoya provides fully validated PhenoCycler Antibodies and a list of supplemental antibodies that have undergone varying levels of testing.

PhenoCycler Inventoried Antibodies

PhenoCycler Inventoried Antibodies are supplied by Akoya and have passed the full PhenoCycler Antibody Validation Process and manufacturing quality control. Our inventoried antibodies are also fully supported by our field support team.

PhenoCycler Antibodies have been tested in multiple types of human FFPE tissue. These PhenoCycler Antibodies are conjugated to a distinct PhenoCycler Barcode (BX000). Each kit contains one tube of PhenoCycler Antibody and one tube of the corresponding dye-labeled Reporter (RX000), sufficient volume for 25 PhenoCycler runs (or tests) at the standard staining conditions. Recommended dyes for FFPE tissue are Atto 550, Cy5, and Alexa Fluor™ 750.
Catalog #
Product
Size
b-Catenin1-BX020 (12F7)—Atto 550-RX020
25 tests
Beta-Actin-BX010(W16197A)—Alexa Fluor™ 488-RX010
25 tests
Beta-Actin-BX010(W16197A)—Alexa Fluor™ 750-RX010
25 tests
CD3e-BX045(EP449E)—Cy5-RX045
25 tests
CD4-BX003 (EPR6855)—Cy5-RX003
25 tests
CD8-BX026 (C8/144B)—Atto 550-RX026
25 tests
CD11c-BX024 (118/A5)—Cy5-RX024
25 tests
CD20-BX007 (L26)—Alexa Fluor™ 488-RX007
25 tests
CD20-BX007 (L26)—Alexa Fluor™ 750-RX007
25 tests
CD21-BX032 (EP3093)—Atto 550-RX032
25 tests
CD31-BX001 (EP3095)—Alexa Fluor™ 488-RX001
25 tests
CD31-BX001 (EP3095)—Alexa Fluor™ 750-RX001
25 tests
CD44-BX005 (IM7)—Atto 550-RX005
25 tests
CD45-BX021(D9M81)—CY5-RX021
25 tests
CD45RO-BX017 (UCHL1)—Atto 550-RX017
25 tests
CD68-BX015 (KP1)—Cy5-RX015
25 tests
CD107a-BX006 (H4A3)—Cy5-RX006
25 tests
E-cadherin-BX014 (4A2C7)—Atto 550-RX014
25 tests
Histone H3 Phospho (Ser28)-BX030 (HTA28)—Cy5-RX030
25 tests
HLA-DR-BX033(EPR3692)—Cy5-RX033
25 tests
IDO1-BX027(V1NC3IDO)—CY5-RX027
25 tests
Keratin 14-BX002(Poly19053)—Atto 550-RX002
25 tests
Ki67-BX047 (B56)—Atto 550-RX047
25 tests
LIF-BX006(M1506B09)—Cy5-RX006
25 tests
Mac2/Galectin-3-BX035(M3/38)—Atto 550-RX035
25 tests
Pan-Cytokeratin-BX019 (AE-1/AE-3)—Alexa Fluor™ 488-RX019
25 tests
Pan-Cytokeratin-BX019 (AE-1/AE-3)—Alexa Fluor™ 750-RX019
25 tests
PCNA-BX036(PC10)—CY5-RX036
25 tests
Podoplanin-BX023 (NC-08)—Atto 550-RX023
25 tests
TFAM-BX029(18G102B2E11)—Atto 550-RX029
25 tests
PhenoCycler Antibodies for human fresh frozen tissue have been tested using tonsil tissue. These PhenoCycler Antibodies are conjugated to a distinct PhenoCycler Barcode (BX000). Each kit contains one tube of PhenoCycler Antibody and one tube of the corresponding dye-labeled Reporter (RX000), sufficient volume for 25 PhenoCycler runs (or tests) at the standard staining conditions. Recommended dyes for human fresh frozen tissue are Atto 550, Cy5, and Alexa Fluor™ 488.
Catalog #
Product
Size
CD2-BX002 (RPA-2.10)—Atto 550-RX002
25 tests
CD3-BX015 (UCHT1)—Cy5-RX015
25 tests
CD4-BX021 (SK3)—Cy5-RX021
25 tests
CD8-BX004 (SK1)—Alexa Fluor™ 488-RX004
25 tests
CD8-BX004 (SK1)—Alexa Fluor™ 750-RX004
25 tests
CD11c-BX027 (S-HCL-3)—Cy5-RX027
25 tests
CD19-BX003 (HIB19)—Cy5-RX003
25 tests
CD21-BX013 (Bu32)—Alexa Fluor™ 488-RX013
25 tests
CD21-BX013 (Bu32)—Alexa Fluor™ 750-RX013
25 tests
CD31-BX032 (WM59)—Atto 550-RX032