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Best practices and guidelines to move your manual assay to your favorite autostainer (Leica Bond RX, Roche Discovery Ultra). We will review Opal Manual workflow at a high level and then delve into details to convert this to an automated workflow using an autostainer. Tips and tricks will be included.
In this webinar Dr. Jonah Cool, Program Officer for Single-Cell Biology at the Chan Zuckerberg Initiative, provides an overview of single-cell efforts at CZI; and Dr. Oliver Braubach, Head of Research Applications at Akoya Biosciences, introduces the CODEX solution and shares case studies on how single-cell, spatial phenotyping applications can push the boundaries of discovery biology in human health and disease.
Immune checkpoint inhibitors are revolutionizing cancer therapy for many. But robust biomarkers are needed to predict which patients will likely benefit from these therapies. A team of investigators at the Mayo Clinic is trying to close this gap by developing a catalog of the human immune system in cancer patients, appropriately called “The Cancer Immunome Project. By analyzing the spatial architecture of FFPE tumor sections across the entire tumor microenvironment, the team is generating a comprehensive map of the cancer immunome. In this webcast, Dr. JC Villasboas shares how his team is using the CODEX® System to deepen our knowledge about the composition, architecture and interactions of the tumor microenvironment.
Hear from Oliver Braubach, Ph.D., Senior Manager, Applications at Akoya Biosciences on the importance of single cell resolution in spatial biology , how to discover single cell contextual phenotypes and the many single-cell, spatial phenotyping applications.
In this presentation, from the 2021 Spatial Biology Europe Congress, Gavin Gordon, Ph.D., Vice President, Clinical Market Development from Akoya Biosciences describes evolving biomarker strategies in immuno-oncology and how multiplexed immunofluorescence has been associated with improved predictive performance. Dr Sebastian Marwitz, ARCN Principal Investigator, Pathology from Research Center Borstel – Leibniz Lung Center, describes how multiplexed IHC has been used for the spatial profiling in lung disease in hypotheses-driven analyses at a cohort level using FFPE patient samples.
Recent studies strongly suggest the importance of determining a patient’s Immunoscore as well as the need for a more comprehensive understanding, both spatially and functionally, of the simultaneous presence of multiple immune cell types within the TME. Immune contexture parameters, including the Immunoscore, have a prognostic, predictive, and mechanistic value. Once identified, the key immune elements should be translated into clinically feasible treatment protocols, integrating with the field of immunopathology. In this webinar, Dr. Jerome Galon describes how a spatial phenotyping approach has supported the development of Immunoscore to estimate the prognosis of colorectal cancer patients
In this multi-part webinar series, our expert speakers review analytical frameworks and algorithms to integrate imaging-based single-cell spatial phenotyping data with complementary transcriptomic and genomic datasets. High-plex cell phenotyping methods like single-cell RNA-seq capture the deep cellular heterogeneity of samples, but cell behavior is a function of all that surrounds it. Imaging-based spatial phenotyping platforms enable researchers to visualize and analyze cell diversity, interactive networks, and cellular behavior across whole tissue sections. Both types of data have complementary features, which give researchers the ability to merge information about a cell’s proteome and transcriptome with its single-cell, spatial context. This webinar series highlights the latest advances driving integrative multiomic analysis.
H&E stromal tumor-infiltrating lymphocyte (sTIL) score and programmed death ligand 1 (PD-L1) SP142 immunohistochemistry assay are prognostic and predictive in early-stage breast cancer but are operator-dependent and may have insufficient precision to characterize dynamic changes in sTILs/PD-L1 in the context of clinical research. In this webinar, the presenters discuss how multiplex immunofluorescence (mIF) combined with statistical modeling can be used to estimate dynamic changes in sTIL score, PD-L1 expression, and other immune variables from a single paraffin-embedded slide, thus enabling comprehensive characterization of activity of novel immunotherapy agents.
In today’s clinical research laboratories, available tissue samples are both smaller in size and more in demand for a variety of assays to help researchers make informed decisions and spatial discoveries. The very cornerstone of tissue histopathology – the H&E stain – is the first crucial step to help map out the morphological landscape. Here, Akoya Biosciences describes a technique in which a tissue sample is stained with a modified H&E stain prior to the application of an Opal Multiplex assay on the same slide, as a screening tool and efficient and effective way to maximize your precious samples.
Dr. Yvonne Saenger, from the Columbia University Herbert Irving Comprehensive Cancer Center, discusses how she used a validated quantitative multiplex immunofluroescence imaging workflow to identify individuals who would respond to adjuvant immunotherapy, better stratifying them into high-risk versus low-risk stage II melanoma.
In this webcast, Dr. Kai Kessenbrock explains how spatial phenotyping can enhance the biological insights from Human Cell Atlas initiatives. Using the Human Breast Cell Atlas project as an example, Dr. Kessenbrock discusses how his team discovered unique cellular niches within the breast tissue microenvironment and how their spatial proximity gives us a more comprehensive view of the biology underlying each sample.
In this talk, Dr. Alexander “Sandy” Borowsky will share preliminary results of an ongoing biomarker discovery study on breast cancer cohorts, including those participating in the I-SPY 2 trial, and how the in situ analysis of the tumor microenvironment is critical to understanding immunotherapy response. The I-SPY 2 trial: Neoadjuvant and Personalized Adaptive Novel Agents to Treat Breast Cancer was launched by UCSF in collaboration with a private-public partnership to improve efficiency of breast cancer clinical trials and streamline the development of new drugs. Breast cancers, overall, infrequently respond to immunotherapy. And immunotherapies, which harness the immune system to fight cancer, are being used in the early high-risk treatment setting. However, individual tumors show dramatic variation in patterns of the tumor immune microenvironment (TIME) with both immune “cold” and inflamed phenotypes. We hypothesized that the TIME pattern might predict responses to immunotherapy and/or prognosis/outcomes. We examined the TIME of high-risk invasive breast cancer and high-risk non-invasive ductal carcinoma in situ (DCIS), hypothesizing that immune evasion might be one of the critical steps in progression of DCIS to invasive carcinoma. Leveraging current clinical trials, we have performed gene expression analyses (bulk) and multiplexed-immunohistochemistry (mIHC). We have optimized two 7-color assay panels using the Opal detection and artificial intelligence-assisted image analysis to provide specific cell segmented assignment of location and applied proximity analyses for cell-cell interaction assessment. These data show distinct differences in the TIME and reveal predictions of early response.
Autofluorescence is a well-known problem associated with multiplex imaging applications, including CODEX. We demonstrate a simple autofluorescence reduction method that integrates readily into the CODEX workflow. The protocol improves CODEX imaging data and streamlines antibody panel design by enabling more reliable use of low wavelength fluorophores, such as Alexa 488.
In this webinar, researchers from the University of Birmingham and the University of Limerick describe how they used multiplexed imaging and single-cell analysis to study the immune response to the SARS-CoV-2 virus across multiple organ systems. Deep immune phenotyping of COVID-19 tissue sections can reveal how the spatial and structural relationships between the virus and different cell types can influence disease pathology and progression.
Optimisation and validation of a multiplex immunofluorescence workflow, from staining to digital image analysis, ensures assay robustness and prepares this technology for deployment in clinical trial analysis and in routine diagnostics. Amélie Viratham, from Queen’s University Belfast, describes how she used the Opal Automation IHC kit and Vectra Polaris to develop two complementary multiplex immunofluorescence panels to evaluate the tumour immune microenvironment in colorectal cancer formalin-fixed paraffin-embedded tissue.
An innovative platform, called AstroPath, has been developed to analyze large multiplex immunofluorescence (mIF) datasets using celestial object–mapping algorithms to rapidly identify optimized predictive phenotypic signatures. In this webinar, Dr. Janis Taube and Dr. Alexander S. Szalay from Johns Hopkins describe how the AstroPath technology was conceived and developed, share successes applying AstroPath to answering questions about tumor microenvironment-immune system interactions, and provide preliminary results of comparisons across different institutions to assess mIF assay performance.
This session covered how researchers are using spatial biology to uncover new biological insights as well as a focus on the CODEX technology and how it will impact your work. This video from the webinar, contains a review of the CODEX technology and how it is used in spatial biology including work on COVID19 and a customer talk on how they have used CODEXs in their work and as a service to others in the academic community as a Core lab service.
This session covered how researchers are using spatial biology to uncover new biological insights as well as the latest innovations from Akoya and how they will impact your work. This video from the webinar, contains a review of the Akoya platforms and how they are used in spatial biology and a customer talk on how they have used Akoya’s platforms in their work on human lung tuberculosis granulomas. A high-level review of selected publications with a focus on how the Akoya platforms can be used on studies beyond immuno-oncology is included.
Dr. Garry Nolan and his team at Stanford University collaborated with the University of Bern to conduct deep single-cell phenotyping and spatial analysis on a cohort of colorectal cancer formalin-fixed, paraffin-embedded samples using the CODEX. In this webinar, Dr. Nolan discusses the findings from the study and presents an analytical framework to analyze high-dimensional imaging data that can reveal new insights into how the tumor microenvironment is organized.
This session covered how researchers are using spatial biology to uncover new biological insights as well as the latest innovations from Akoya and how they will impact your work. This video includes both customer talks on how they have used Akoya’s platforms in their immune-oncology work and a high-level discussion of the impact of spatial biology on current research and its promise for future work.
Neuroscience forum with Akoya’s neuroscientists Agnes Haggerty, PhD, Technical Applications Scientist, Najiba Mammadova, PhD, Reagent Sales Specialist and Oliver Braubach, PhD, Senior Manager Applications. Discussions on how multispectral analysis can impact neuroscience research by studying the spatial relationships between cells and tissue types using a large number of biomarkers simultaneously and how the Akoya platform mitigates the confounding effects of autofluorescence. A closer review of how to use the Opal staining platform to study any biomarker and how the CODEX platform can support ultra-high plex analysis with 25 or more biomarkers at once.
Proxima™, The Spatial Biology Platform™ enables rapid image acquisition and secure data sharing in the cloud with immediate access from anywhere for geographically distributed and remote teams, including those working from home during the COVID-19 pandemic. Learn more about how Proxima will improve the efficiency of your research in this webinar.
Coronavirus disease 2019 (COVID-19) is a new pandemic disease caused by infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). A multi-institutional exploratory research taskforce, named EXPLORE COVID-19, conducted a longitudinal analysis of immune responses of patients at various stages of COVID-19 severity. The goal of the study, which also included a team from Innate Pharma, was to gain translational insights to better understand the immune response in COVID-19 patients and identify potential targets to fight the viral infection. During this webinar, Dr. Remark will provide an overview of the study methodology. Thereafter, he will focus on the C5a-C5aR1 axis and explain its role in the pathophysiology of acute respiratory distress syndrome. This was done by using several techniques, including multiplexed immunohistochemistry. Together, the data support C5a-C5aR1 axis blockade as a means of limiting myeloid cell infiltration in damaged organs and preventing the excessive lung inflammation and endothelialitis in COVID-19 patients. The results from this study were applied to an ongoing COVID-19 clinical trial.
Marquee webinar featuring the recent neuroscience work, published in Cell, by Dr. Ke-qi Fan, Research Lead, Jin Jin Molecular Immunology Lab, Life Sciences Institute of Zhejiang University
Rakuten Medical Inc. Principal Scientist, Chung-Wein Lee, PhD, describes the workflow outlined in his recently published manuscript in the Journal of Immunological Methods. Dr. Lee explains how his team used Akoya’s multispectral imaging platform and Opal staining kits to reliably interrogate the tumor microenvironment in large B-cell lymphoma, something that was previously a challenge.
NeoGenomics lead scientist for Vectra Polaris studies, Sara Pollan, PhD, explains the process to successfully optimize and validate a custom 7-color opal panel for multiplex immunofluorescence using the Vectra Polaris platform, to successfully profile and quantify exhausted T-cells in head and neck squamous cell carcinoma.
Boston University, Assistant Professor of Pathology and Laboratory Medicine, Jonathan Cherry, PhD, speaks about how the opal staining platform and the Vectra Polaris imaging system enabled him to study the immune profile of diseased brain tissue, a historically challenging tissue type in terms of autofluorescence. Specifically, Dr. Jon Cherry discusses his work studying the evolution of neuronal and glial tau isoforms in Chronic Traumatic Encephalopathy.
AstraZeneca, Associate Principal Scientist, Michael Surace, PhD, speaks about his experience as an early user of Proxima, Akoya’s cloud-based image analysis and collaboration software. Dr. Mike Surace also discusses multispectral immunofluorescence workflows, bottlenecks, and advantages of Proxima.
Akoya Biosciences, Technical Applications Scientist, Agnes Haggerty, PhD, explains the importance of multispectral immunofluorescence and multispectral imaging, and how to get as much information from your precious tissue samples as possible. Dr. Agnes Haggerty provides a high-level overview of Akoya’s Phenoptics platform, our high throughout multiplex immunohistochemistry and immunofluorescence paired with machine learning analysis.
Recorded on September 28, 2020. Featuring speakers from Kyushu University, Enable Medicine, and Akoya Biosciences, this webinar introduces the capabilities of the CODEX system to uncover novel insights from intact tissue sections and focuses on graft-versus-host disease (GVHD) of the gut as a case example
Recorded on September 24, 2020. Featuring speakers from Immunomic Therapeutics and Precision for Medicine, this webinar explores the clinical utility of CTCs and CTC biomarker data and methods to effectively capture, image, and analyze CTCs. A case study in melanoma is presented, investigating whether CTCs could serve as an effective PD biomarker to support an adaptive trial design
Recorded on September 15, 2020. In this webinar, you’ll learn about the importance of multiplex biomarker analysis including how advances in multiplexed immunofluorescent imaging and analysis can reveal biological and spatial information.
Akoya Academy Webinar recorded on August 25, 2020. Technical Applications Scientist, Clemens Duerrschmid, PhD, speaks about the development and validation of a high throughout 9 color immunofluorescence assay for translational studies in immune oncology research.
Bethyl Labs, Inc. Director of IHC and Digital Pathology, Mike Spencer, PhD, speaks about strategies to optimize and validate antibody panels for multiplex immunofluorescence. In this video, Mike introduces Bethyl Labs’ PathPlex® antibody panels, which are optimized for Akoya’s Phenoptics™ instrument platform.
Texas Children’s Hospital and Baylor College of Medicine Assistant Professor David Steffin, MD, speaks about his experiences with Akoya’s CODEX® system in studying the microenvironment of pediatric tumors. Here, he describes his workflow and ways to analyze CODEX® data to generate high-impact, meaningful, results.
Heather Stevenson-Lerner, MD, PhD, FCAP, Director of Transplantation Pathology at The University of Texas Medical Branch, speaks about multispectral imaging in her daily research, and how it enabled her to characterize intrahepatic macrophages in patients with Chronic Liver Disease. In this video, Dr. Stevenson-Lerner introduces her recent article in Hepatology Communications.
Akoya Technical Application Scientist Clemens Duerrschmid, PhD, speaks about the importance of multiplex biomarker analysis. In this video, we show, how multiple layers of biological and spatial information can be obtained from a single sample with Akoya’s Phenoptics™ and CODEX® platforms.
Recorded on July 14, 2020. Reagent Sales Specialist, Najiba Mammadova, PhD, and Technical Applications Scientist, Agnes Haggerty, PhD, cover the new PD-1/PD-L1 MOTiF™ Panel kits and rules based inForm™ phenotyping. Learn about fully automated 6-Plex, 7 color on the BOND RX, streamlined Vectra Polaris imaging, and see phenotyping in action with an inForm demo.
During this customer webinar, we demonstrate a novel in situ detection strategy to better characterize the inflammatory burden during kidney and cardiac allograft rejection by targeting NK cells, T lymphocytes and macrophages; and to better localize those cells within or outside the microcirculation.
Recorded on May 28, 2020. In this webinar, Dr. Eliot Peyster from the University of Pennsylvania outlines the potential of in-situ immune-phenotyping for improving diagnostic accuracy and rejection risk stratification.
Recorded on May 19, 2020. Senior Field Application Scientist, Mahdis Rahmani, provides tips on how to optimize CODEX images for use in presentations and scientific literature.
Recorded on May 12, 2020. Senior Applications Scientist, Oliver Braubach, PhD, explains how to perform single-cell analysis of cell suspensions with the CODEX workflow. He also provides a demo of the MAV gating function using PBMCs as an example.
Speakers from Stanford and Akoya will introduce the CODEX System as a pragmatic solution for small- to medium-scale single-cell analyses of cell suspensions, using PBMCs…
Learn about Cell Signaling Technology’s antibody validation techniques for single and multiplex applications, the importance of species-specific validation, and the advantages of rabbit monoclonal antibodies in […]
Iterative multiplex immunohistochemistry (IHC) can be challenging. In this webinar, we’ll introduce two new fully optimized and ready-to-use Opal MOTiF 7-color I/O panels for full automation staining. We’ll discuss basic tips and tricks for customization as well as the pre-analytical considerations to make every stain a success.
In this webinar, expert speakers from the Fred Hutchinson Cancer Research Center will discuss how they successfully implemented an automated, multiplexed tissue imaging platform for spatial profiling of the TME.
In this webinar, Diane Saunders, a staff scientist in the Vanderbilt Diabetes Center, will describe the validation process for custom panels of CODEX antibodies using lightly fixed tissue samples.
In partnership, Akoya® and Visiopharm® , have partnered to develop the VIS APP “Multiplexed Immunofluorescent, Immune Infiltrate Analysis” for the analysis of multiplexed immunofluorescent slides stained with an immuno-oncology biomarker panel that have been unmixed using inForm® Tissue Finder.
In this webinar, we discuss how Akoya Biosciences Phenoptics assays support quantitative mIF to overcome limitations imposed by conventional IHC methodologies. We also discuss how our Opal assay kits and reagents can be integrated with the Leica BOND RX to automate your staining workflow to support consistent results for high-throughput studies.
After tissue analysis, researchers are left with large, unwieldy data tables that are difficult to interpret. phenoptrReports provides a simple-to-use data analysis tool that permits analysis of complex phenotypes and their spatial interactions. This webinar provides a live demo of phenoptrReports’ functionality:
Proscia and Akoya Biosciences, two industry-leading multispectral analysis companies, share the exciting new ways their platforms impact your high-throughput analysis workflows.
In this webinar you’ll learn about our comprehensive multiplexed tissue imaging solutions spanning biomarker discovery and clinical research and validation. With its high multiplexing capability, CODEX is ideally suited for biomarker discovery. Phenoptics is the industry standard for high throughput multiplex IHC and ideally suited for translational research.
Dr. Garry Nolan of Stanford University presents evidence of deep internal order in immune functionality demonstrating that differentiation and immune activities have evolved with a definable “shape”. This webcast will demonstrate CODEX (CO-Detection by indEXing) as the next-generation fluorescent imaging technology to provide highly multiplexed analysis of tissues.
Recorded on Thursday, March 26, 2020 Speakers: Oliver Braubauch, PhD, Senior Applications Scientist, Akoya Biosciences Abstract: The CODEX technology combines the advantages of modern single-cell omics technologies with…