Opal 7-Color IHC Kits
Opal™ 7 color kits provide a practical workflow for the simultaneous detection of up to eight tissue biomarkers plus nuclear counterstain within a single image. The method is similar to standard immunohistochemistry (IHC) and is accessible to many laboratories where standard IHC is performed.
Catalog Number |
Name | Link to Shop Online |
size |
NEL811001KT | Manual IHC Kit | 50 slides |
NEL821001KT | Automated IHC Kit | 50 slides |
Product Details
The Manual Opal 7-Color IHC Kit contains:
- 6 reactive fluorophores (Opal 520, Opal 540, Opal 570, Opal 620, Opal 650 and Opal 690)
- Spectral DAPI
- 1 X Plus Amplification Diluent
- Blocking/Ab Diluent
- Opal Polymer HRP Ms + Rb
- AR6 Buffer
The Automated Opal 7-Color IHC Kit contains:
- 6 reactive fluorophores (Opal 520, Opal 540, Opal 570, Opal 620, Opal 650 and Opal 690)
- Spectral DAPI
- 1 X Plus Automation Amplification Diluent
- Blocking/Ab Diluent
- Opal Polymer HRP Ms + Rb
Users should source primary antibodies and other workflow reagents separately. The Opal 7-Color Manual IHC Kit was optimized for use on the Vectra® and Mantra™ systems. Kits contain enough reagents to stain 50-slides at the recommended 1:100 or 1:150 dilution ratio.
Opal provides researchers with the tools to interrogate multiple pathways while retaining context provided by tissue images. This approach provides information not available from alternative techniques like flow cytometry or analysis of single markers in serial sections.
Opal will enable you to:
- Measure up to six tissue biomarkers at once
- Use the best primary antibodies together in multiplex panel, with no species-based crosstalk
- Retain spatial cellular context that is lost in flow cytometry
- Confirm single cell co-expression for many biomarkers in one tissue section
- Get more information while conserving precious tissue.
Specifications
Shipping Condition | Blue Ice |
Sample | Human |
Product Category | Opal Kits |
Type | Opal Detection Kit |
Detection Method | Mantra and Vectra Quantitative Pathology Imaging Instruments |
Workflow
- Bake at 60 C for one hour
- Xylene wash (3x ten minutes)
- Rehydrate with EtOH gradient into di H2O
- 10% NBF for 20 minutes
- Di H2O wash
- AR6 or AR9 MWT
- Cool to RT for at least 15 minutes
- Di H2O rinse, TBST rinse
- PAP pen barrier
- Incubate tissue for 10 minutes in blocking solution at RT
- Remove blocker and add primary antibody
- Rinse with TBST, then wash 3 times for two minutes each in TBST
- Incubation 10 minutes at RT
- Rinse with TBST and wash 3 times for 2 minutes each
- Opal fluorophore incubation for ten minutes at RT
- Rinse with TBST and wash 3 times for 2 minutes each
- Rinse slides with AR6 or AR9
- Place slides in microwave-safe jar, perform MWT
- Cool to RT for at least 15 minutes
- Use AR6 for MWT prior to DAPI for removal of any unbound fluor
- Rinse slides in di H2O and then TBST
- Incubate DAPI for 5 minutes at RT
- Wash with di H2O and TBST two minutes each
- Apply monunting medium for fluorescene microscopy for coverslip
Documents
Find instructions for use below.
Download Staining Manual
Download Manual IHC Data Sheet
Download Automated IHC Data Sheet
Material Safety Data Sheets
Catalog Number |
Name |
MSDS |
NEL811001KT | Manual IHC Kit | Download MSDS [English] Download MSDS [Japanese] |
NEL821001KT | Automated IHC Kit | Download MSDS [English] Download MSDS [Japanese] |