TSA Plus DIG
TSA Plus DIG uses horseradish peroxidase (HRP) to catalyze covalent deposition of digoxigenin (DIG) labels directly adjacent to the immobilized enzyme. The labeling reaction is quick (less than 10 minutes) and deposited labels can then be detected using anti-digoxigenin conjugates for fluorescent or bright field microscopy.
Each TSA Plus DIG kit contains the following 2 components necessary for signal amplification:
DIG Amplification Reagent and 1X Plus Amplification Diluent.
The use of TSA Plus reagents results in a significant increase in sensitivity over standard detection methods, while maintaining specificity and resolution. Moreover, TSA Plus reagents allow drastically reduced consumption of primary antibody or probe.