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PhenoCode Signature Immuno-Contexture Human Protein Panel
Tumors characterized as "hot" exhibit signs of inflammation, high T cell infiltration, and expression of PD-L1. These inflamed tumors typically respond well to checkpoint inhibitor immunotherapy. PhenoCode Signature Immuno-Contexture panel (CD8, CD68, PD-L1, FoxP3, PanCK) enables profiling of the tumor microenvironment for CD8+ T lymphocytes and PD-L1 expression. Map additional phenotypes by adding a 6th marker to the panel from the antibody list below or add your own marker of interest by using the Antibody Conjugation Kit to barcode your antibody of choice.
Step 1: Order Panel
Catalog #
Product
Size
PCSPP001
PhenoCode Signature Immuno-Contexture Human Protein Panel
(CD8, CD68, PD-L1, FoxP3, PanCK)
(CD8, CD68, PD-L1, FoxP3, PanCK)
Up to 20 Slides
Step 2: Choose 1 Antibody
Catalog #
Product
Size
S6501003
Anti-Hu CD3e(AKYP0125)-BX080 for PhenoCode Signature
Up to 20 Slides
S6501006
Anti-Hu Ki67(AKYP0126)-BX047 for PhenoCode Signature
Up to 20 Slides
S6501008
Anti-Hu CD20(AKYP0049)-BX064 for PhenoCode Signature
Up to 20 Slides
S6501009
Anti-Hu Granzyme B(AKYP0086)-BX041 for PhenoCode Signature
Up to 20 Slides
OR Barcode your own Antibody
Catalog #
Product
Size
PCSP0200
Antibody Conjugation Kit for PhenoCode Signature
Up to 20 Slides
Step 3: Detect your +1 Antibody with Opal 520
Catalog #
Product
Size
FP1487001KT
Opal 520 Reagent Pack
Up to 50 Slides
Step 1: Order Panel
Catalog #
Product
Size
PCSPP001
PhenoCode Signature Immuno-Contexture Human Protein Panel
(CD8, CD68, PD-L1, FoxP3, PanCK)
(CD8, CD68, PD-L1, FoxP3, PanCK)
Up to 20 Slides
Step 2: Choose 1 Antibody
Catalog #
Product
Size
S6501003
Anti-Hu CD3e(AKYP0125)-BX080 for PhenoCode Signature
Up to 20 Slides
S6501006
Anti-Hu Ki67(AKYP0126)-BX047 for PhenoCode Signature
Up to 20 Slides
S6501008
Anti-Hu CD20(AKYP0049)-BX064 for PhenoCode Signature
Up to 20 Slides
S6501009
Anti-Hu Granzyme B(AKYP0086)-BX041 for PhenoCode Signature
Up to 20 Slides
OR Barcode your own Antibody
Catalog #
Product
Size
PCSP0200
Antibody Conjugation Kit for PhenoCode Signature
Up to 20 Slides
Step 3: Detect your +1 Antibody with Opal 520
Catalog #
Product
Size
FP1487001KT
Opal 520 Reagent Pack
Up to 50 Slides
Product Details
Literature
Educational Resources
Product Details
PhenoCode Signature panels are validated for the PhenoImager platform which integrates staining, imaging, and analysis using well-established workflows while providing accuracy and reproducibility. Featuring a flexible design component, PhenoCode Signature panels allow for the easy integration of a novel checkpoint or immune cell marker into a 5-plex base panel.
PhenoCode Signature Immuno-Contexture Human Protein Panel contains:
- 5 Primary antibodies targeting CD8, CD68, FoxP3, PD-L1, and Pan-Cytokeratin
- 5 PhenoCode Signature Detectors
- 5 Opal dyes + DMSO for signal amplification: Opal 480, Opal Opal 570, Opal 620, Opal 690, Opal 780 + TSA-DIG
- 10X Spectral DAPI
- 1X Plus Automation Amplification Diluent
- PhenoCode Signature Diluent
- 1X Antibody Diluent/Block
- PhenoCode Signature Blocker 1
- PhenoCode Signature Blocker 5
- N Blocker
- J Blocker
- S Blocker
Literature
Optimization Guide
Guide for Optimization of PhenoCode Signature Assays and Panels
Technical Note
Coming Soon
PhenoCode Signature Immuno-Contexture Human Protein Panel
SDS
Coming Soon
Educational Resources
Coming Soon
