Opal 7 Solid Tumor Immunology Kit

The Opal™ 7 Solid Tumor Immunology Kit enables phenotyping of helper T cells, cytotoxic T cells, B cells, macrophages and cancer cells within intact FFPE tissue sections.

Catalog Number
Name | Link to Shop Online
Size
OP7TL4001KT Opal 7 Solid Tumor Immunology Kit 50 slides

Product Details

The Opal™ 7 Solid Tumor Immunology Kit enables phenotyping of helper T cells, cytotoxic T cells, B cells, macrophages and cancer cells within intact FFPE tissue sections.

The Opal™ 7 Solid Tumor Immunology Kit includes all critical workflow reagents to enable phenotyping of helper T cells, cytotoxic T cells, B cells, macrophages and cancer cells within intact FFPE tissue sections using 6-plex fluorescent immunohistochemistry:

  • Primary antibodies (anti-CD4, anti-CD8, anti-CD20, anti-CD68, antiFoxP3 and anti-Pan Cytokeratin)
  • Antibody diluent
  • Fluorescent IHC detection reagents
  • Spectral DAPI
  • Antigen retrieval buffers

This kit was configured and validated for multispectral imaging on the Vectra and Mantra Systems. It contains enough reagents for 6-plex immunostain of 50 slides.

  • Optimized panel for assessment of immune cells in intact FFPE tumor tissue.
  • Shortest path to results
  • Part of the Phenoptics™ workflow for immunophenotyping of solid tumors

Specifications

Shipping Condition Blue Ice
Sample Human
Product Category Opal Kits
Type Opal Detection Kit
Detection Method Mantra and Vectra Quantitative Pathology Imaging Instruments

Workflow

Bake and Deparaffinize Slides
  • Bake at 60 C for one hour
  • Xylene wash (3x ten minutes)
  • Rehydrate with EtOH gradient into di H2O
Slide Fixation
  • 10% NBF for 20 minutes
  • Di H2O wash
Antigen Retrieval
  • AR6 or AR9 MWT
  • Cool to RT for at least 15 minutes
Blocking
  • Di H2O rinse, TBST rinse
  • PAP pen barrier
  • Incubate tissue for 10 minutes in blocking solution at RT
Primary Antibody
  • Remove blocker and add primary antibody
  • Rinse with TBST, then wash 3 times for two minutes each in TBST
Secondary Antibody
  • Incubation 10 minutes at RT
  • Rinse with TBST and wash 3 times for 2 minutes each
Opal Reagent
  • Opal fluorophore incubation for ten minutes at RT
  • Rinse with TBST and wash 3 times for 2 minutes each
MWT
  • Rinse slides with AR6 or AR9
  • Place slides in microwave-safe jar, perform MWT
  • Cool to RT for at least 15 minutes
Spectral DAPI
  • Use AR6 for MWT prior to DAPI for removal of any unbound fluor
  • Rinse slides in di H2O and then TBST
  • Incubate DAPI for 5 minutes at RT
  • Wash with di H2O and TBST two minutes each
Mount
  • Apply monunting medium for fluorescene microscopy for coverslip

Documents

Phenoptics Portflolio
Brochure
Learn about our complete solution.

Download

Opal Assay Development Guide
User Manual
Comprehensive user manual for Opal staining.

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Opal 7 Color Solid Tumor Immunology Multiplex IHC Assay
Data Sheet

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