We’ve rebranded some of our products, learn more ›
CODEX is now PhenoCycler
Phenoptics is now Phenolmager
Reagents Support
We’re here to support your multiplexed imaging needs.
Reagents for PhenoCycler
Find the documents you need to get started with the PhenoCycler™ workflow.
- User Manual: PhenoCycler User Manual
- Quick Reference Card: Antibody Conjugation Workflow
- Quick Reference Card: FF Tissue Staining and Reporter Plate Preparation
- Quick Reference Card: FFPE Tissue Staining and Reporter Plate Preparation
- Guidelines: Antibody Screening and Custom Conjugation – Tips and Guidelines
- Guidelines: Inventoried Antibody Dilutions for PhenoCycler
- Guidelines: Tissue Processing – Best Practices
- Guidelines: Investigation of Autofluorescence – Best Practices
Need help with troubleshooting? Check out the FAQs or contact technical support
Opal and TSA Reagents Support

Get tips on assay development using the comprehensive assay development guide.
- Assay Development Guide: Assay Development Guide
- Quick Reference Guide: Akoya Opal™ Pairings with Primary Antibodies
- Publication: Validation of multiplex immunofluorescence panels using multispectral microscopy for immune-profiling of formalin-fixed and paraffin-embedded human tumor tissues
- Publication: Eight-Color multiplex immunohistochemistry for simultaneous detection of multiple immune checkpoint molecules within the tumor microenvironment.
- Akoya MOTiF™ 6-plex Mouse Tissue Analysis BOND RX Protocol
- Akoya MOTiF 6-plex Checkpoint Proliferation BOND RX Protocol
- Akoya 8-plex Field of View Slide Staining BOND RX Protocol
Support Documentation
- IHC Guidebook: Sixth Edition from Dako: This comprehensive educational guidebook on the best practices for pre-analytics, staining, and image analysis published by Dako gives in-depth guidance on achieving successful and accurate staining.
- Publication: Antibody validation
- Publication: Garbage in, garbage out: a critical evaluation of strategies used for validation of immunohistochemical biomarkers.
Contact Technical Support
Akoya products are fully supported by our experienced technical support personnel. We are here to help whenever you need us.