Reagents Support
Reagents Support
CODEX Reagents
Find the documents you need to get started with the CODEX workflow.
- User Manual: CODEX User Manual
- Quick Reference Card: Antibody Conjugation Workflow
- Quick Reference Card: FF Tissue Staining and Reporter Plate preparation
- Quick Reference Card: FFPE Tissue Staining and Reporter Plate preparation
- Guidelines: Antibody Screening and Custom Conjugation – Tips and Guidelines
- Guidelines: CODEX Inventoried Antibodies Dilutions
- Guidelines: Tissue Processing – Best Practices
- Guidelines: Investigation of Autofluorescence – Best Practices
Need help with troubleshooting? Check out the FAQs or contact technical support
Opal and TSA Reagents Support
Get tips on assay development using the comprehensive assay development guide.
- Assay Development Guide: Assay Development Guide
- Quick Reference Guide: Akoya Opal Pairings with Primary Antibodies
See how others have developed and optimized their multiplex IHC assays using Opal reagents.
- Publication: Validation of multiplex immunofluorescence panels using multispectral microscopy for immune-profiling of formalin-fixed and paraffin-embedded human tumor tissues
- Publication: Eight-Color Multiplex Immunohistochemistry for Simultaneous Detection of Multiple Immune Checkpoint Molecules within the Tumor Microenvironment.
Automated Multiplex IHC Staining Protocols
- Akoya MOTiF™ 6-plex Mouse Tissue Analysis BOND RX Protocol
- Akoya MOTiF™ 6-plex Checkpoint Proliferation BOND RX Protocol
- Akoya 8-plex Field of View Slide Staining BOND RX Protocol
Technical Note
If you have any questions about assay development or need help with troubleshooting, search the FAQs or contact technical support.
Support Documentation
- IHC Guidebook: Sixth Edition from Dako: This comprehensive educational guidebook on the best practices for pre-analytics, staining, and image analysis published by Dako gives in-depth guidance on achieving successful and accurate staining.
- Publication: Antibody validation
- Publication: Garbage in, garbage out: a critical evaluation of strategies used for validation of immunohistochemical biomarkers.