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PhenoCode Signature M1/M2 Polarization Human Protein Panel
Tumor associated macrophages (TAMs) play a key role in regulating the inflammatory or immunosuppressive state of the tumor microenvironemnt (TME). Distinguish between M1 (pro-inflammatory) and M2 (anti-inflammatory) TAMs using the PhenoCode™ Signature M1/M2 Polarization panel (CD8, CD68, CD163, PD-1, PD-L1) to evaluate polarization profiles in the TME. Map additional phenotypes by adding a 6th marker to the panel from the antibody list below or add your own marker of interest using the Antibody Conjugation Kit to barcode your antibody of choice.
Step 1: Order Panel
Catalog #
Product
Size
PCSPP004
PhenoCode Signature M1/M2 Polarization Human Protein Panel
(CD8, CD68, CD163, PD-1, PD-L1)
(CD8, CD68, CD163, PD-1, PD-L1)
Up to 20 Slides
Step 2: Choose 1 Antibody
Catalog #
Product
Size
S6501000
Anti-Hu Pan-Cytokeratin (AKYP0053)-BX066 for PhenoCode Signature
Up to 20 Slides
S6501002
Anti-Hu CD4 (AKYP0048)-BX003 for PhenoCode Signature
Up to 20 Slides
S6501003
Anti-Hu CD3e (AKYP0125)-BX080 for PhenoCode Signature
Up to 20 Slides
S6501006
Anti-Hu Ki67 (AKYP0126)-BX047 for PhenoCode Signature
Up to 20 Slides
S6501007
Anti-Hu FoxP3 (AKYP0102)-BX031 for PhenoCode Signature
Up to 20 Slides
S6501008
Anti-Hu CD20 (AKYP0049)-BX064 for PhenoCode Signature
Up to 20 Slides
S6501009
Anti-Hu Granzyme B (AKYP0086)-BX041 for PhenoCode Signature
Up to 20 Slides
S6501012
Anti-Hu CD45RO (AKYP0059)-BX017 for PhenoCode Signature
Up to 20 Slides
S6501013
Anti-Hu SMA (AKYP0081)-BX013 for PhenoCode Signature
Up to 20 Slides
OR Barcode your own Antibody
Catalog #
Product
Size
PCSP0200
Antibody Conjugation Kit for PhenoCode Signature
Up to 20 Slides
Step 3: Detect your +1 Antibody with Opal 520
Catalog #
Product
Size
FP1487001KT
Opal 520 Reagent Pack
Up to 50 Slides
PhenoCode Signature panels are validated for the PhenoImager® platform which integrates staining, imaging, and analysis using well-established workflows providing accuracy and reproducibility. Featuring a flexible design element, PhenoCode Signature panels allow for the easy integration of a novel checkpoint or immune cell marker into a 5-plex base panel.
PhenoCode Signature M1/M2 Polarization Human Protein Panel contains:
- 5 Primary antibodies targeting CD8, CD68, CD163, PD-1, and PD-L1
- 5 PhenoCode Signature Detectors
- 5 Opal dyes + DMSO for signal amplification: Opal 480, Opal 570, Opal 620, Opal 690, Opal 780 + TSA-DIG
- 10X Spectral DAPI
- 1X Plus Automation Amplification Diluent
- PhenoCode Signature Diluent
- 1X Antibody Diluent/Block
- PhenoCode Signature Blocker 1
- PhenoCode Signature Blocker 5
- N Blocker
- J Blocker
- S Blocker
PhenoCode Signature M1/M2 Polarization Human Protein Panel
Comprehnsive Document Lisitng Kit Components, Material & Methods to run the M1/M2 Polarization Human Protein Panel
Technical Data Sheet & Protocol
Six Plex for Success
Strategies for Developing Clinically Useful Spatial Signature Biomarkers
White Paper
Decoding the Science of Response to Cancer Therapy
Benefits of PhenoCode Signature Panels and Case Study
Application Note
A Comprehensive Approach to Immunotherapy Response Prediction: Unlocking Spatial Signatures with Complementary PhenoCode Signature Panels
Poster
Identifying a role for macrophages in predicting immunotherapy responses of non-small cell lung cancer (NSCLC)
Poster
