Tissue staining with the opal polaris 7 colour ihc detection kit

Opal Polaris 7 Color IHC Detection Kits

Opal™ Polaris Kits are part of a practical workflow for the simultaneous detection of up to eight tissue biomarkers plus nuclear counterstain within a single image, across the entire digital slide. The method is similar to standard immunohistochemistry (IHC) and is accessible to many laboratories where standard IHC method development is performed. Opal Polaris 7-Color IHC Detection Kits are optimized for whole slide multispectral imaging with the Vectra® Polaris™ Automated Quantitative Pathology Imaging Instrument.

Catalog Number
Name | Link to Shop Online
Size
NEL861001KT Opal Polaris 7-Color Manual IHC Detection Kit 50 slides
NEL871001KT Opal Polaris 7-Color Automated IHC Detection Kit 50 slides

Product Details

The Opal Polaris 7-Color Automation IHC Detection Kit is part of the Phenoptics 2.0 whole slide workflow and contains:

  • 6 reactive fluorophores (Opal Polaris 480, Opal 520, Opal 570, Opal 620, Opal 690, and Opal Polaris 780)
  • Spectral DAPI
  • 1X Plus Automation Amplification Diluent
  • Blocking/Ab Diluent
  • Opal Polymer HRP Ms + Rb
  • Users should source primary antibodies and other workflow reagents separately. The Opal Polaris 7-Color Automation IHC Kit was optimized for use on the Leica Biosystems BOND RX and configured and validated for imaging on the Vectra® Polaris™ quantitative pathology imaging system. Kits contain enough reagents to stain 50-slides at the recommended 1:150 dilution ratio.

 

The Opal Polaris 7-Color Manual IHC Detection Kit is part of the Phenoptics 2.0 whole slide workflow and contains:

  • 6 reactive fluorophores (Opal Polaris 480, Opal 520, Opal 570, Opal 620, Opal 690, and Opal Polaris 780)
  • Spectral DAPI
  • 1X Plus Amplification Diluent
  • Blocking/Ab Diluent
  • Opal Polymer HRP Ms + Rb
  • AR6 Buffer
  • Users should source primary antibodies and other workflow reagents separately. The Opal 7-Color Manual IHC Kit was optimized for use on the Vectra® Polaris™ quantitative pathology imaging system. Kits contain enough reagents to stain 50-slides at the recommended 1:100 dilution ratio.

Specifications

Shipping Condition Blue Ice
Sample Human
Product Category Opal Kits
Type Opal Detection Kit
Detection Method Mantra and Vectra Polaris Quantitative Pathology Imaging Instruments

Documents

Phenoptics Portflolio
Brochure
Learn about our complete solution.

Download

Opal Assay Development Guide
User Manual
Comprehensive user manual for Opal staining.

Download

Opal Polaris 7 Color Multiplex IHC Assay
Data Sheet

Download Manual Kit Data Sheet
Download Automated Kit Data Sheet

Cloud-Based Image Analysis and Collaboration is Here.

X