Unlocking Single Cell Biology in FFPE Tissues

In this video, Oliver Braubach, PhD, Senior Applications Scientist at Akoya Biosciences, demonstrates how the Multiplex Analysis Viewer (MAV) software can be used to analyze FFPE tissue samples with single-cell, spatial resolution. We look at an example of breast cancer FFPE tissue stained with a 36-antibody CODEX® panel.
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    Whole Slide Multispectral Imaging | A Guided Tour of a 7 Color MOTIF™ Image with Cliff Hoyt

    Mini-semina with Cliff Hoyt, VP of Translational and Clinical Programs at Akoya Biosciences, showing how the MOTIF™ technology can unmix an entire digital slide and how this reveals the biology. This particular sample is a melanoma FFPE slide stained with the Opal Polaris 7 Color Kit.

    Value of Autofluorescence Isolation Using MOTiF™ Whole Slide Multispectral Technology

    Mini-seminar where Cliff Hoyt, VP of Translational and Clinical Programs at Akoya Biosciences, shows the importance of unmixing and isolating autofluorescence from your multiplexed samples. This lymphoma biopsy sample was stained using the Opal Polaris 7 Color Reagent Kit.

    Next Generation Cancer Treatment: Patient Stratification with Immuno-Oncology

    This brief video explains how using biomarkers on tissue samples not only provides new insights into the biology of cancer but ultimately enables a new level of patient stratification.  This allows the most effective treatment to be used for any given cancer leading to significantly improved outcomes for patients.

    Whole Slide Multiplex Tissue Analysis with Vectra Polaris System

    True multiplex tissue analysis enables multiple biomarkers to be identified and spatial relationships between different cell types to be analyzed. The Vectra Polaris System provides a high throughput solution from Opal staining to whole slide imaging and multi-parameter image analysis for a deeper understanding of biology.

    Bernard Fox, Ph.D. Discusses SITC Immunoscore Validation Project

    Bernard Fox, Ph.D., Harder Family Endowed Chair in Cancer Research at the Earle A. Chiles Research Institute, discusses key finding from the SITC Immunoscore Validation Project.  The ability to clearly discriminate between tumors with or without immune cell infiltrates is critical to properly stratifying patients for interventional treatments.

    Impact of Multiplex Tissue Analysis in Immuno-oncology Research

    Lisa Butterfield, Ph.D., President of the Society of Immunotherapy of Cancer, Bernard Fox, Ph.D. Harder Family Endowed Chair in Cancer Research and Clifford Hoyt, MS, Vice President of Translational and Clinical Programs at Akoya Biosciences discuss the impact of multiplex tissue analysis on immuno-oncology research and translational studies.

    Opal Multiplex IHC Assay Development and Optimization Tutorial

    In this short video, Akoya Biosciences® Opal Applications Leader, Virginie Goubert, walks us through the streamlined Opal Multiplex Fluorescent IHC assay development and optimization process. Learn to speed up development time by following this simplified experimental design, leading to balanced signals, full dynamic expression of each marker, and faster quantitative results.

    Multispectral Imaging Explained

    This brief overview shows the power of multispectral imaging to unlock the full potential of spatial biology. This includes a discussion of the confounding effects of cross-talk between spectra and how this limitation has been successfully addressed with spectral-unmixing.