We’ve rebranded some of our products, learn more ›
CODEX® is now PhenoCycler,
Phenoptics™ is now Phenolmager.
PhenoImager HT
Providing unparalleled speed, performance, and throughput, the PhenoImager® HT instrument running the new HT 2.0 software integrates the power of multispectral imaging with onboard spectral unmixing in a simplified workflow enabling spatial signatures at scale.
Product Details
Key Benefits
Specifications
Literature
Education Resources
Product Details
As the premier and most highly cited imager for spatial phenotyping and spatial signature development, the PhenoImager HT is the fastest whole-slide multispectral imaging system that can be easily integrated into high-throughput workflows to accommodate for scalability. PhenoImager HT running the newly released 2.0 software provides researchers with a unique technology stack combining onboard spectral unmixing, rapid imaging and manageable data outputs. When combined with PhenoCode Signature assays for 6-plex, 7 Color whole slide imaging, HT 2.0 delivers unparalleled performance for spatial signature development.
Key Benefits
- State of the art multispectral imaging with onboard spectral unmixing enables the identification and quantification of multiple overlapping biomarkers (up to 6) without the interference of autofluorescence and signal cross-talk
- Renowned liquid crystal tunable filter (LCTF) technology generates unmixed, annotated regions of interest of up to 9-colors for deeper interrogation of biology
- Generate whole slide scans of up to 7 colors in less than 12 minutes (15mm x 15mm region) for analysis of biology across the entire slide in a streamlined workflow and without selection bias
- Fully automated, high-throughput system to accelerate your research and maximize your lab’s resources
- High speed digital whole-slide scanning at 10X-40X in brightfield or fluorescence
- Enclosed system with built-in touchless automation allows you to visualize, analyze, quantify and phenotype immune cells in situ with enhanced security and reliability
- Integrated inForm and phenoptr tissue analysis software packages support configurable projects for biomarker quantification and spatial analysis
Specifications
| Tissue Formats | Tissue microarrays and tissue sections |
| Multispectral Range | 440 – 780 nm |
| Modality | Brightfield and Fluorescence (multispectral or color) |
| 40x Throughput: Fluorescence (1.5 x 1.5 cm) | 3 digital slides per hour (7 color) |
| 40x Throughput: Brightfield (1.5 x 1.5 cm) | 8 digital slides per hour |
| Automation | Touchless, with walk-away image acquisition |
| Slide capacity | 80 slide capacity with continuous loading technology |
| Multiplexing Capability | Separates up to 9 colors, even if overlapping |
| Image Analysis Software | inForm: intuitive learn-by-example interface to automatically segment and quantitate tissue structures, cells and sub-cellular signatures |
| Resolution | 40x = 0.25 μm/pixel 20x = 0.5 μm/pixel 10x = 1.0 μm/pixel |
| File Format | Akoya Biosciences’ whole slide scan image (.qptiff); Multispectral field format (.iM3); monochrome or color images (JPEG, single-layer TIFF, BMP, or PNG) |
| Product Brand Name | PhenoImager HT |
| Automation Compatible | Yes |
| Detection Method | Fluorescence, Brightfield |
| Light Source | LED |
Literature
Education Resources
Related Products
PhenoCycler-Fusion 2.0
Rapid, deep spatial phenotyping
PhenoImager Fusion
Spatial Signatures Within Reach
PhenoCode Signature Assays
Predesigned Panels for PhenoImager
