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Uncovering Novel Immune Landscapes in Ovarian Cancer with Spatial Proteomics

Webinar | Tuesday, May 14, 2024

Survival rates for patients with high grade serous carcinoma of the ovary (HGSC) have stagnated for the past fifty years. Despite the presence of tumor mutations and infiltration of immune cells, existing immunotherapies have yielded limited efficacy against HGSC. These observations highlight a gap in the understanding of how the immune system functions and interacts within HGSC tumors. 
To address this gap, a comprehensive analysis of duplicate core samples from 939 HGSC patients was performed. Leveraging high parameter immunohistochemical/Opal multiplex staining, digital pathology, computational biology, and multivariate analysis, immune cell subsets and their associations with HGSC tumors were identified. 
In this webinar, the speakers will discuss their findings including six patterns of cellular infiltration defined by spatially restricted unsupervised clustering of cell subsets. These patterns were represented in most patient samples, but their specific distributions differed. Notably, higher infiltration of CD16a+ cells, along with their co-localization with macrophages, T cells, and NK cells within specific cellular neighborhoods, corresponded to improved overall (OS) and progression-free survival (PFS) of HGSC patients. 
Learning Objectives:
  • Define key considerations when optimizing immunofluorescence panels for multiplex imaging and spatial analysis 
  • How to validate tissue and cellular segmentation, and staining patterns in collaboration with pathologists 
  • Evaluate strategies towards developing experiment and hypothesis-specific spatial analysis algorithms for ovarian and other cancers 




Riley Arseneau

Riley Arseneau, BSc

Dalhousie University

Riley Arseneau, BSc
Riley Arseneau (she/her) is a PhD Student investigating the correlations between genetics, natural killer cells, and pancreatic cancer in the Boudreau lab at Dalhousie University. She is supported by the Terry Fox MOHCCN Health Informatics and Data Science Award, and the Dalhousie Faculty of Medicine and BHCRI CRTP graduate studentships. Riley earned her BSc from Dalhousie University.

Sarah Nersesian

Sarah Nersesian, MSc

Dalhousie University
Sarah Nersesian, MSc 
Sarah Nersesian (she/her) is a PhD candidate investigating the relationships between immune natural killer cells and ovarian cancer in the Boudreau lab at Dalhousie University with support from Killam and Vanier scholarships. Sarah obtained her BSc from the University of Guelph and her MSc from Queen’s University studying the multifaceted features that allow cancer to metastasize.