Cancer research and pathology relies heavily on formalin-fixed, paraffin embedded (FFPE) tissues from patients. While FFPE samples are easy and cost-effective to store, they are often incompatible with contemporary omics techniques, like single-cell sequencing.
The CODEX® platform overcomes this limitation. It’s compatible with FFPE tissues and can image 40+ protein biomarkers with single-cell resolution, while retaining spatial context. The Akoya team developed and tested the following antibody panel to study breast cancer in human FFPE tissue using CODEX. This ultra-high plex, 36-antibody panel contains mostly epithelial markers targeted at different secretory and non-secretory epithelia in breast tissue, as well as cell state and proliferation markers that are frequently implicated in cancer etiology.