Vectra 3 Automated Quantitative Pathology Imaging System

The Vectra® 3 automated quantitative pathology imaging system can accelerate your cancer immunology research.

The Vectra 3 automated quantitative pathology imaging system, together with the inForm® software, provides multispectral and automated multi-slide imaging of up to 6 slides to detect and measure weakly expressed and overlapping biomarkers in H&E, IHC or IF intact FFPE tissue sections or TMAs within a familiar digital pathology workflow.

Product Details

Vectra 3, used with the Phenochart™ whole slide contextual viewer, facilitates annotation capability into the digital pathology workflow, where pathologists and technicians can navigate around slides and identify areas of interest for high-resolution multispectral acquisition. It accurately measures protein and biomarker expression and morphometric characteristics in intact tissue sections for translational studies.

Key Benefits

  • Supports Akoya’s Phenoptics™ workflow solution for cancer immunology research
  • Detects and measures multiple weakly expressed and overlapping biomarkers within a single H&E, IHC or IF tissue section and in TMAs
  • Visualizes, analyzes, quantifies and phenotypes immune cells in situ in solid tissues
  • Automated loading of up to 6 slides
  • Phenochart whole slide viewer for slide navigation provides context for identification of regions of interest within a digital pathology workflow
  • Automatic identification of specific tissue types using integrated inForm analysis software


Tissue Formats Tissue microarrays (TMAs) and tissue sections
Spectral Range 440 – 720nm (tunable)
Modality Brightfield and Fluorescence (multispectral or color)
Scan time (1.5 cm x 1.5 cm sample, plus the acquisition of 10 MSI fields at 20x) 5-band Fluorescence at 10x = 18 minutes
5-band Fluorescence at 4x = 12 minutes
Brightfield at 10x = 10 minutes
Throughput for TMAs 5 cores (0.6mm) per minute in Brightfield
Multiplexing Capability Separates up to 7 colors, even if overlapping
Image Analysis Software inForm, two seat licenses
Resolution 10x = 1 um per pixel
4x = 2.5 um per pixel
File Format .QPTIFF (Akoya Biosciences whole slide scans); .iM3 (multispectral fields); and JPEG/BMP/PNG (color images)
Product Brand Name Vectra 3
Automation Compatible Yes
Detection Method Fluorescence, Brightfield
Light Source LED

Support Documents

Phenoptics Portflolio


Understanding immune phenotypes and their spatial relationships to breast adenocarcinoma in FFPE tissues


Detecting Phenotypical Subgroups in Breast Cancer using Multiplexed Protein Expression Analysis in Intact Tissue Sections
App Note