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During this webinar, Dr. Jessica Da Gama Duarte from Olivia Newton-John Cancer Research Institute will present the protocol for the in situ interrogation and characterization of TLSs in human and murine tissue sections using Opal-tyramide signal amplification multiplex immunohistochemistry. This protocol enables simultaneous detection of up to 7 markers (6 antigens and a DAPI counterstain). She will also describe a grading system to identify immature and mature TLSs.
Key learning objectives
Dr Jessica Da Gama Duarte obtained a PhD in Medical Biochemistry at the University of Cape Town, South Africa in 2015, where she developed a novel protein microarray-based tumour antigen array. Her aims were to use this technology to identify novel diagnostic, prognostic and predictive cancer biomarkers. She was recruited as a Postdoctoral Research Fellow by the Olivia Newton-John Cancer Research Institute, Australia in the Cancer Immunobiology Program. This Institute provides a direct link between research and clinical practice, and access to a suite of patient samples that enable her to investigate the full translational capacity of the array.
She is currently working on research projects that are aiming towards early detection of cancer onset and recurrence, prediction of immunotherapy benefit and/or toxicity, and understanding the role of B cells in anti-tumour immunity.
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