In today’s clinical research laboratories, available tissue samples are both smaller in size and more in demand for a variety of assays to help researchers make informed decisions and spatial discoveries.

The very cornerstone of tissue histopathology – the H&E stain – is the first crucial step to help map out the morphological landscape. Here, Akoya Biosciences describes a technique in which a tissue sample is stained with a modified H&E stain prior to the application of an Opal™ Multiplex assay on the same slide, as a screening tool and efficient and effective way to maximize your precious samples.

You will learn:

• Not all H&E is created equal. See how simulated H&E allows for superior morphological context
• The benefits of viewing H&E and multiplex stains on the same tissue.
• How to make the most out of your valuable tissue sections—conduct mIF and H&E on the same slide without using serial sections or tissue alignment software
• How Opal staining complements standard H&E analysis with a more quantitative and precise methodology.