Leaps in imaging, mass spectrometry-based proteomics, protein engineering, and chemical probe technologies have empowered study of the proteome at increasingly smaller resolutions. Instead of associating protein abundance at the organ or cell level with normal and disease physiology, it is possible to understand how subcellular trafficking and localization contribute to pathology. With the advancement of these technologies, several new approaches can be used to achieve a spatial view of the proteome. In this webinar, Kathryn Lilley (University of Cambridge), Garry Nolan (Stanford University), and Jeffrey Spraggins (Vanderbilt University School of Medicine) will discuss the opportunities and challenges of using spatial proteomics to tackle basic research to disease biology.