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Beyond Cell Densities: Spatially Resolved Methods to Analyze Tumor Immune Microenvironment from Multiplex Immunofluorescence Images

Webinar | September 27, 2023

The tumor immune microenvironment plays a pivotal role in cancer development, progression, and metastasis. With the advent of immunotherapy, there is an urgent need to understand the biology underlying tumor–immune interactions.

Multiplex Immunofluorescence (mIF) has been demonstrated to be an invaluable tool to analyze the tumor immune microenvironment. MFI allows the simultaneous identification of multiple tumoral, immune, and non-immune cell types. Data analysis of mIF experiments can be challenging due to the diversity of cell types and the complexity of spatial configurations present in the immune microenvironment.

In this webinar, we will explore how mIF can help researchers to analyze the tumor-immune interface. We will focus on state-of-the-art analytical methods to analyze mIF images. In addition to basic immune cell quantification methods, we will discuss the analysis of other microenvironment characteristics like intra-tumor heterogeneity and spatial cellular neighborhood identification.




Alvaro Lopez Janeiro

Alvaro López Janeiro

Department of Pathology. Clínica Universidad de Navarra

Álvaro López-Janeiro  earned his medical degree from the University of Navarra and completed his residency at University Hospital La Paz (Madrid, Spain). As a pathologist and scientist, Álvaro helps to develop platforms to study molecular biomarkers for prediction of response or resistance to immunotherapy and mechanisms of immune evasion. He was involved in a pivotal study that showed neoadjuvant nivolumab modifies the tumor immune microenvironment in resectable glioblastoma. Currently, his main focus is the study of gynecological cancer patients - developing new biomarkers to predict relapse and response to immunotherapies.

Amelie Viratham

Amélie Viratham

Technical Applications Scientist, Akoya Biosciences

Amélie Viratham - In her previous role as Research Associate in the Tissue Hybridisation & Digital Pathology group at Queen’s University Belfast, Amélie utilised Akoya’s PhenoImager products to investigate the tumour immune microenvironment in epithelial cancers. Amélie has since taken up the role as Reagents Field Application Scientist at Akoya, where she supports scientists in using Akoya’s technologies to study spatial biology at single-cell resolution, with a focus on reagents, staining and biomarker panel development.