Authors: Nagle, Maeve P.; Tam, Gabriela S.; Maltz, Evan; Hemminger, Zachary; Wollman, Roy
Issue: Cell Syst. 2021 May 19;12(5):388-400.
Biological organization crosses multiple spatial scales: from molecular, cellular, to tissues and organs. The proliferation of molecular proﬁling technologies enables increasingly detailed cataloging of the components at each scale. However, the scarcity of spatial proﬁling has made it challenging to bridge across these scales. Emerging technologies based on highly multiplexed in situ proﬁling are paving the way to study the spatial organization of cells and tissues in greater detail. These new technologies provide the data needed to cross the scale from cell biology to physiology and identify the fundamental principles that govern tissue organization. Here, we provide an overview of these key technologies and discuss the current and future insights these powerful techniques enable.