Events

Akoya Biosciences at The Society for Immunotherapy of Cancer (SITC) Annual Meeting 2019
 

November 6 – 10, 2019 | National Harbor, MD | Booth # 330
 

We offer a suite of powerful tools for spatially resolved, multiplexed tissue imaging that can unravel the complexities of the tumor microenvironment. Join us at SITC 2019 to learn about the latest developments on our CODEX® and Phenoptics™ platforms.


Join us for an evening of cocktails and conversations on the latest developments in spatial biology

Title: High-plex Spatial Analysis of the Tumor Microenvironment: Advancements and Applications
Date and time: Friday, November 8, 2019, 6:30 to 8:00 PM
Venue: Riverview B4&5, Gaylord National Convention Center, National Harbor

Register for Dinner Symposium

 

AGENDA:

Simplifying the mIHC workflow with new Opal™ MOTiF 7-Color immuno-oncology panel kits
Victoria Duckworth, Product Manager, Multiplex IHC Assays and Reagents, Akoya Biosciences, Inc.

 

Cross-validating biomarkers on the CODEX® and Phenoptics™ platforms
Cliff Hoyt, VP Translational Programs and Scientific Affairs, Akoya Biosciences, Inc.
Julia Kennedy-Darling, PhD, Director, R&D, Akoya Biosciences, Inc.

 

Leveraging multiplex IHC to explore the role of myeloid cells in enforcing immunosuppression in the tumor microenvironment
Robert Pierce, MD, Scientific Director, Immunopathology lab, Fred Hutchinson Cancer Research Center, USA

 

Introductions: Brian McKelligon, CEO and Terry Lo, President, Akoya Biosciences, Inc.

 

DESCRIPTION:

Recent studies have demonstrated the critical importance of studying the spatial architecture of the tumor microenvironment (TME) to develop predictive biomarkers for checkpoint inhibitors (Lu et al, JAMA Oncology, Jul 2019). As ‘The Spatial Biology Company™’, we aim to empower researchers with comprehensive tools that can span applications across biomarker discovery, translational research and clinical trials. Our CODEX® benchtop platform integrates with third-party fluorescence microscopes to image up to 40 markers per tissue sample, making it ideal for hypothesis-free biomarker discovery. Our Phenoptics™ solution, which includes the Vectra® and Mantra™ family of instruments, Opal™ IHC staining kits, and inForm® image analysis software, is the industry standard for multiplexed IHC (mIHC)/multiplexed immunofluorescence (mIF) and can image up to 8 markers per tissue and up to 80 slides per run. The platform is currently being used by leading cancer research centers to conduct high throughput clinical research studies.

Attend our workshop to learn about the latest innovations in the CODEX® and Phenoptics™ workflows and how researchers can translate their findings across these two platforms for biomarker profiling in tumor samples. In the first talk, we will give an overview of two novel, ready-to-use Opal™ immuno-oncology panels which will streamline user optimization and downstream image analysis, and are validated on melanoma and lung cancer samples. Thereafter, we will present the preliminary results of a study comparing shared sets of immune and tumor markers between the CODEX® and Phenoptics™ platforms. This cross-platform comparison will provide a conceptual framework for researchers to translate biomarker signatures from discovery to high throughput translational studies. And finally, Dr. Rob Pierce, MD, will review how his team at the Fred Hutchinson Cancer Research Center uses our multiplexed immunofluorescence platforms to interrogate the complexities of the TME and how they correlate with prognosis and treatment outcomes.


POSTERS

Stop by our posters to see the latest data from our platforms and for a chance to have an in-depth discussion with our R&D and application scientists.

 

#P64: HIGHLY MULTIPLEXED SINGLE-CELL SPATIAL ANALYSIS OF FFPE TUMOR TISSUES USING CODEX®
Saturday, Nov. 9, 7:00 am - 8:30 pm
Jessica Yuan1, Gajalakshmi Dakshinamoorthy1, Joseph Kim1, Sejal Mistry1, Nadya Nikulina1, Roya Bashier1, Cassandra Hempel1, Maria Elena Gallina1, Julia Kennedy-Darling1

1 Akoya Biosciences, Inc. 1505 O’Brien Drive Suite A-1, Menlo Park, CA, 94025

 

#P43: A NOVEL PLATFORM FOR HIGHLY MULTIPLEXED, SINGLE-CELL IMAGING OF CELL SUSPENSIONS
Friday, Nov. 8, 7:00 am - 8:00 pm
Anum Khan1, Won-Mean Lee3, Dhananjay Wagh2, John Coller2, Gabriel Mercado3, Jon Mulholland1

1 Cell Sciences Imaging Facility (CSIF), Stanford University, CA, USA,
2 Stanford Functional Genomics Facility, Stanford University, CA, USA,
3 Akoya Biosciences, Menlo Park, CA, USA

 

#P31: APPLYING MULTISPECTRAL UNMIXING AND SPATIAL ANALYSES TO EXPLORE TUMOR HETEROGENEITY WITH A PRE-OPTIMIZED 7-COLOR IMMUNO-ONCOLOGY WORKFLOW
Friday, Nov. 8, 7:00 am - 8:00 pm
Carla Coltharp1, Bethany Remeniuk1, Chichung Wang1, Yi Zheng1, Rachel Schafer1, Linying Liu1, Glenn Milton1, Carla Coltharp1, Victoria Duckworth1, Michael McClanev, Peter Miller1 and Yi Zheng1

1 Akoya Biosciences, Hopkinton, MA, USA

 

#P34: A FULLY OPTIMIZED END-TO-END SOLUTION FOR I/O MULTIPLEX IMMUNOFLUORESCENCE STAINING USING OPAL POLARIS 7-COLOR PD1/PD-L1 PANEL KITS FOR LUNG CANCER AND MELANOMA
Saturday, Nov. 9, 7:00 am - 8:30 pm
Yi Zheng1, Rachel Schaefer1, Linying Liu1, Glenn Milton1, Carla Coltharp1, Victoria Duckworth1, Michael McLane1, and Peter Miller1

1 Akoya Biosciences, Hopkinton, MA, USA

 

#P54: DEVELOPMENT OF A 9-COLOR IMMUNOFLUORESCENCE ASSAY USING TYRAMIDE SIGNAL AMPLIFICATION AND MULTISPECTRAL IMAGING FOR HIGH-THROUGHPUT STUDIES ON FFPE TISSUE SECTIONS
Saturday, Nov. 9, 7:00 am - 8:30 pm
Bethany Remeniuk1, Carla Coltharpv, Kristin Roman1, Chichung Wang1, and Clifford Hoyt1

1 Akoya Biosciences, Hopkinton, MA, USA

Our application scientists will be hosting in-booth software demos (booth #330) during the ‘Lunch, Exhibits and Poster Viewing’ sessions on Friday, Nov 8, 12:30 - 2:00 pm, and Saturday, Nov 9, 12:50 – 1:50 pm. Get an in-depth, hands-on demo of the following software packages:

  • Akoya inForm® and phenoptrReports: Analyzing and visualizing complex phenotypes. Report spatial metrics with ease. 
  • Visiopharm APP (Just released!): Automated immune infiltrate analysis of unmixed mIF imagery
  • IndicaLab HALO™: Whole slide analysis of unmixed mIF imagery

FEATURED PRODUCTS

The CODEX System
 

For hypothesis-free biomarker discovery

  • Images 40 protein markers in a tissue sample
  • Compact and budget-friendly
  • Preserves the sample for downstream ROI analysis or H&E staining

The Vectra® Polaris™ System

For hypothesis-driven biomarker discovery and high throughput clinical trials

  • MOTiF™ whole-slide multispectral imaging of up to 80 slides per run
  • Unmixing technology enables precise quantitation of protein markers
  • Image up to 8 markers in a tissue sample

Opal™ Ready-to-use Panels and IHC Staining Kits

  • Off-the-shelf immuno-oncology panels to simplify assay implementation
  • Optimized for manual and automated staining workflows

inForm® Tissue Finder™ and phenoptrReports

  • Automated image analysis and segmentation
  • Powerful unmixing engine to separate weak, spectrally overlapping markers
  • Advanced spatial analysis reporting