This post is part of a two-part series on Akoya’s software tools for spatial analysis. Part 2 covers the inForm Tissue Analysis software.
With the right analysis tools, researchers can move from multiplexed imaging data to visualizing the spatially resolved cellular structure and cellular phenotypes of the tissue microenvironment. As part of the CODEX® solution for spatially resolved, multiplexed immunofluorescence, the CODEX software suite includes powerful, easy-to-use tools for spatial biomarker analysis.
The CODEX Software Analysis Suite is comprised of three core components:
- Data Collection — The CODEX Instrument Manager (CIM) is used to control the fluidics instrument and integrates with the microscope control software for image acquisition
- Data Processing — The CODEX Processor formats images for downstream analysis and is used to perform drift compensation, background subtraction, deconvolution, cell segmentation and clustering
- Data Interpretation — The Multiplex Analysis Viewer (MAV) is an ImageJ plugin used to visualize, annotate, and analyze cell populations
Since we’re focusing on the analysis capabilities of the CODEX software suite in this post, we’ll cover some of the main features of the Multiplex Analysis Viewer (MAV).