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Tumor Infiltrating Lymphocyte 6-Plex Panel
formerly Opal 7 Tumor Infiltrating Lymphocyte Kit
The Tumor Infiltrating Lymphocyte 6-Plex Panel enables phenotyping of T cells, B cells and cancer cells within intact FFPE tissue sections. Kit is ideal for studying immune cell infiltration of tissues including tumors.
Catalog #
Product
Size
Product Details
Specifications
Workflow
Literature
Data Sheets
Education Resources
Product Details
The Tumor Infiltrating Lymphocyte 6-Plex Panel includes all critical workflow reagents to enable phenotyping of T cells, B cells and cancer cells within intact formalin-fixed, paraffin-embedded tissue sections using 6-plex fluorescent immunohistochemistry:
- Primary antibodies (anti-CD4, anti-CD8, anti-CD20, anti-CD45RO, anti-FoxP3 and anti-Pan-Cytokeratin
- 1X Antibody Diluent/Block
- 6 reactive fluorophores (Opal 520, Opal 540, Opal 570, Opal 620, Opal 650, and Opal 690)
- DMSO
- 10X Spectral DAPI
- 1X Plus Manual Amplification Diluent
- Secondary Antibody 1X Opal Anti-Ms + Rb HRP
- AR6 Buffer
- AR9 Buffer
This kit was configured and validated for multispectral imaging on the Mantra and PhenoImager HT Systems. It contains enough reagents for 6-plex immunostain of up to 50 slides.
- Optimized panel for assessment of TILs in intact FFPE tissue.
- Shortest path to results
- Part of the PhenoImager® workflow for immunophenotyping of solid tumors
Specifications
| Shipping Condition | Blue Ice |
| Sample | Human |
| Product Category | Opal Kits |
| Type | Opal Detection Kit |
| Detection Method | Mantra 2, Vectra 3, and PhenoImager HT |
Workflow
Bake and Deparaffinize Slides
- Bake at 60 C for one hour
- Xylene wash (3x ten minutes)
- Rehydrate with EtOH gradient into di H2O
Slide Fixation
- 10% NBF for 20 minutes
- Di H2O wash
Antigen Retrieval
- AR6 or AR9 MWT
- Cool to RT for at least 15 minutes
Blocking
- Di H2O rinse, TBST rinse
- PAP pen barrier
- Incubate tissue for 10 minutes in blocking solution at RT
Primary Antibody
- Remove blocker and add primary antibody
- Rinse with TBST, then wash 3 times for two minutes each in TBST
Secondary Antibody
- Incubation 10 minutes at RT
- Rinse with TBST and wash 3 times for 2 minutes each
Opal Reagent
- Opal fluorophore incubation for ten minutes at RT
- Rinse with TBST and wash 3 times for 2 minutes each
MWT
- Rinse slides with AR6 or AR9
- Place slides in microwave-safe jar, perform MWT
- Cool to RT for at least 15 minutes
Spectral DAPI
- Use AR6 for MWT prior to DAPI for removal of any unbound fluor
- Rinse slides in di H2O and then TBST
- Incubate DAPI for 5 minutes at RT
- Wash with di H2O and TBST two minutes each
Mount
- Apply monunting medium for fluorescene microscopy for coverslip
Literature
Opal Storage and Stability
Storage, reconstitution and dilution of Opal dyes.
Technical Brief
Data Sheets
Education Resources
