The mechanisms mediating lymph node metastasis have remained largely unclear until the emergence of spatial analysis. Spatial biology analysis technologies such as Akoya’s PhenoCycler®-Fusion have played a critical role in understanding key cellular interactions involved in lymph node metastasis, disease progression, and tissue organization. In this on-demand webinar, the speakers will discuss how PhenoCycler-Fusion enables fast, single-cell multiplexed imaging of 100+ antibodies as well as 100+ RNA molecules across whole-slide FFPE samples collected from a large cohort of patients with primary tumor, lymph nodes with metastasis and controls. Ultrahigh-plex protein and RNA analysis facilitated the discovery of critical cell-cell interactions and molecular cues necessary for further metastasis. In addition, the ability to measure both protein and RNA targets in a scalable manner across entire tissue sections makes any spatial biology question accessible.