Webinar Category: Phenolmager (Phenoptics)

Association of COVID-19 inflammation with activation of the C5a-C5aR1 axis

Coronavirus disease 2019 (COVID-19) is a new pandemic disease caused by infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). A multi-institutional exploratory research taskforce, named EXPLORE COVID-19, conducted a longitudinal analysis of immune responses of patients at various stages of COVID-19 severity. The goal of the study, which also included a team from Innate Pharma, was to gain translational insights to better understand the immune response in COVID-19 patients and identify potential targets to fight the viral infection. During this webinar, Dr. Remark will provide an overview of the study methodology. Thereafter, he will focus on the C5a-C5aR1 axis and explain its role in the pathophysiology of acute respiratory distress syndrome. This was done by using several techniques, including multiplexed immunohistochemistry. Together, the data support C5a-C5aR1 axis blockade as a means of limiting myeloid cell infiltration in damaged organs and preventing the excessive lung inflammation and endothelialitis in COVID-19 patients. The results from this study were applied to an ongoing COVID-19 clinical trial.

Multiplex Immunofluorescence Application in Diffuse Large B cell Lymphoma

Rakuten Medical Inc. Principal Scientist, Chung-Wein Lee, PhD, describes the workflow outlined in his recently published manuscript in the Journal of Immunological Methods. Dr. Lee explains how his team used Akoya’s multispectral imaging platform and Opal staining kits to reliably interrogate the tumor microenvironment in large B-cell lymphoma, something that was previously a challenge.

Profiling Exhausted T Cells using Vectra® Polaris™ Multiplex Immunofluorescence Assay

NeoGenomics lead scientist for PhenoImager™ HT (formerly Vectra® Polaris™) studies, Sara Pollan, PhD, explains the process to successfully optimize and validate a custom 7-color opal panel for multiplex immunofluorescence using the PhenoImager HT platform, to successfully profile and quantify exhausted T-cells in head and neck squamous cell carcinoma.

Evolution of Neuronal and Glial Tau Isoforms in Chronic Traumatic Encephalopathy (CTE)

Boston University, Assistant Professor of Pathology and Laboratory Medicine, Jonathan Cherry, PhD, speaks about how the opal staining platform and the Vectra Polaris imaging system enabled him to study the immune profile of diseased brain tissue, a historically challenging tissue type in terms of autofluorescence. Specifically, Dr. Jon Cherry discusses his work studying the evolution of neuronal and glial tau isoforms in Chronic Traumatic Encephalopathy.

Phenoptics™ and CODEX®: High Content Multiplexed Imaging for Translational and Discovery Research

Akoya Biosciences, Technical Applications Scientist, Agnes Haggerty, PhD, explains the importance of multispectral immunofluorescence and multispectral imaging, and how to get as much information from your precious tissue samples as possible. Dr. Agnes Haggerty provides a high-level overview of Akoya’s Phenoptics platform, our high throughout multiplex immunohistochemistry and immunofluorescence paired with machine learning analysis.

Webinar: Instant Gratification: Multiplex IF in a Snap with Mantra

Instant Gratification: Multiplex IF in a Snap with Mantra

Akoya Academy Webinar recorded on August 25, 2020. Technical Applications Scientist, Clemens Duerrschmid, PhD, speaks about the development and validation of a high throughout 9 color immunofluorescence assay for translational studies in immune oncology research.

Houston User Group Meeting: Multispectral Imaging Enables Characterization of Intrahepatic Macrophages in Patients With Chronic Liver Disease

Heather Stevenson-Lerner, MD, PhD, FCAP, Director of Transplantation Pathology at The University of Texas Medical Branch, speaks about multispectral imaging in her daily research, and how it enabled her to characterize intrahepatic macrophages in patients with Chronic Liver Disease. In this video, Dr. Stevenson-Lerner introduces her recent article in Hepatology Communications.