In this webinar, we discuss how an innovative multiomic solution can redefine the way we think about spatial phenotyping, the infinite possibilities these techniques provide for spatial biology research.
Webinar Category: PhenoCycler (Codex)
Spatial phenotyping relies on whole-side imaging at single-cell resolution to visualize and quantitate biomarker expression and reveal how cells interact and organize across the tissue landscapes. Watch this 3-part webinar serios to explore how PhenoCycler-Fusion system enables rapid, multiomic spatial phenotyping to uncover novel biological insights.
This webinar highlights the power of CODEX multiplex imaging for surveying the cellular diversity of the human kidney, with potential applications in pathology, histology, and building anatomical atlases. Dr. Elizabeth K. Neumann, from Vanderbilt University, describes the use of co-detection by indexing (CODEX) multiplexed immunofluorescence for visualizing 23 antigens within the human kidney. Using CODEX, many of the major cell types and substructures, such as collecting ducts, glomeruli, and thick ascending limb, were visualized within a single tissue section. Of these antibodies, 19 were conjugated in-house, demonstrating the flexibility and utility of this approach for studying the human kidney using traditional antibody markers.
In this webinar, we’ll share how phenoptrReports provides powerful, easy-to-use tools to analyze spatial relationships between cellular phenotypes and visualize their relationships overlaid on the tissue, phenotype cells co-expressing markers, determine the quality of your unmixing library, and display results in shareable, easy-to-read data reports. Tips and tricks for getting the most out of your analysis will be reviewed as well.
Observing cell-cell interactions is critical to understanding causative factors of biological outcomes. As the only benchtop platform capable of multiplexing >40 markers in tissue, CODEX® (rebranded to PhenoCycler™) uniquely enables these observations by assaying tissue biopsies using fluidics, an inverted microscope, oligo-conjugated antibodies, and fluorescent reporters.
In this webinar Dr. Jonah Cool, Program Officer for Single-Cell Biology at the Chan Zuckerberg Initiative, provides an overview of single-cell efforts at CZI; and Dr. Oliver Braubach, Head of Research Applications at Akoya Biosciences, introduces the CODEX solution and shares case studies on how single-cell, spatial phenotyping applications can push the boundaries of discovery biology in human health and disease.
Immune checkpoint inhibitors are revolutionizing cancer therapy for many. But robust biomarkers are needed to predict which patients will likely benefit from these therapies. A team of investigators at the Mayo Clinic is trying to close this gap by developing a catalog of the human immune system in cancer patients, appropriately called “The Cancer Immunome Project. By analyzing the spatial architecture of FFPE tumor sections across the entire tumor microenvironment, the team is generating a comprehensive map of the cancer immunome. In this webcast, Dr. JC Villasboas shares how his team is using the CODEX® System to deepen our knowledge about the composition, architecture and interactions of the tumor microenvironment.