Immuno-Oncology

SITC 2021: Spatial Biology Poised to Address Challenges in Immuno-Oncology

Studies in recent years have established that the spatial arrangements of cells hold information crucial to understanding immunotherapy response. In a study published in the Journal for ImmunoTherapy of Cancer (JITC), Lu et al. found that multiplex imaging-based approaches like multiplex immunohistochemistry (mIHC) and immunofluorescence (mIF) had much higher predictive power compared to single-plex PD-L1 IHC or single-cell sequencing. Those findings were reflected at this year’s Society for Immunotherapy of Cancer (SITC) Annual Meeting…

Webinar Rewind: Pushing the Frontiers of CAR T-Cell Therapy

Pediatric cancer is an urgent and important problem that requires highly precise diagnostics and therapeutic approaches, however many of the necessary tools have yet to be developed. Around 60% of pediatric cancers are solid tumors, and the prognosis of these cancers is poor with mortality as high as 40%. Advanced solid tumors are highly complex and extremely successful at evading the immune response. Dr. David Steffin, a pediatric oncologist and assistant professor…

Multi-Institutional Efforts to Standardize Multiplex Immunofluorescence for Spatial Biomarker Analysis

In the era of immunotherapy, it's essential to match the right patient to the right treatment. Effective biomarkers are needed to enable patient stratification and ensure that more patients benefit from precision medicine. While many novel techniques have been developed to aid scientists in their hunt for better immunotherapy biomarkers, spatial phenotyping has emerged as …

Multi-Institutional Efforts to Standardize Multiplex Immunofluorescence for Spatial Biomarker Analysis Read More »

Multispectral Multiplex Immunofluorescence: Laying the Groundwork for Clinical Testing in Immuno-Oncology

There is a pressing need for tests that can accurately predict response to immunotherapies, writes Cliff Hoyt, our VP of Translational & Scientific Affairs, in a review article for Frontiers in Molecular Biosciences. Established methods are limited in the amount of information they can pull out of formalin- fixed, paraffin-embedded (FFPE) tissue sections. Conventional immunohistochemistry …

Multispectral Multiplex Immunofluorescence: Laying the Groundwork for Clinical Testing in Immuno-Oncology Read More »

From astronomy to pathology with AstroPath

From Stars to Cells: Johns Hopkins Researchers Discover Predictive Spatial Phenotypic Signatures with AstroPath

One of the biggest questions in immuno-oncology is figuring out how patients will respond to immunotherapy. Approaches like single-plex immunohistochemical stains don’t capture enough information to accurately predict response. To help solve this challenge, researchers at Johns Hopkins University (JHU) developed AstroPath, a novel platform which combines the Phenoptics multiplex immunofluorescence (mIF) workflow with sky-mapping …

From Stars to Cells: Johns Hopkins Researchers Discover Predictive Spatial Phenotypic Signatures with AstroPath Read More »

Astronomy & Pathology Join Forces to Predict Immunotherapy Response: Q&A with Spatial Biology Experts

Researchers at Johns Hopkins University (JHU) recently published findings from AstroPath, the result of a unique collaboration between the astrophysics and pathology departments at JHU. The platform enables deep whole-slide imaging and spatial profiling of microscopic tumor sections by combining the Phenoptics™ platform for multiplex immunofluorescence with sky-mapping algorithms derived from the Sloan Digital Sky …

Astronomy & Pathology Join Forces to Predict Immunotherapy Response: Q&A with Spatial Biology Experts Read More »

Spatial Biology Takes Center Stage at AACR 2021: Part 2 of our conference recap

The AACR Annual Meeting went virtual for the second year in a row in 2021. The cancer research community has done an admirable job of adapting to the new format – AACR brought together leaders in the field to share new research and discuss the future of cancer treatment. While we miss meeting our users …

Spatial Biology Takes Center Stage at AACR 2021: Part 2 of our conference recap Read More »

Spatial Biology Takes Center Stage at AACR 2021: Part 1 of our conference recap

The AACR Annual Meeting went virtual for the second year in a row in 2021. The cancer research community has done an admirable job of adapting to the new format – AACR brought together leaders in the field to share new research and discuss the future of cancer treatment. While we miss meeting our users …

Spatial Biology Takes Center Stage at AACR 2021: Part 1 of our conference recap Read More »

Melanoma tissue stained with 6-plex multiplex immunofluorescence panel

Webinar Rewind: Astronomy Meets Pathology

What does astronomy have to do with biomarker discovery? At Johns Hopkins, pathologists and astronomers collaborated to develop AstroPath, an innovative approach which analyzes large multiplex IF datasets with celestial object–mapping algorithms to identify optimized predictive phenotypic signatures. In a recent webinar, Janis Taube, MD, MSc, Director of the Dermatopathology Division and Fellowship and Alexander …

Webinar Rewind: Astronomy Meets Pathology Read More »