Resource: Poster

Analysis of FFPE Human Tumor Tissues Using PhenoCycler (formerly CODEX) with Signal Amplification

We expanded our CODEX (now rebranded as PhenoCycler) workflow to amplify the fluorescent signal intensities of low expressing biomarkers by combining Tyramide Signal Amplification (TSA) with CODEX, also known as CODEX® Amp. In this poster, we present the analysis of CODEX data for a panel of over 20 biomarkers on human FFPE tissue, including signal …

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Developing and Validating a High-throughput 9-color Immunofluorescence Assay for Translational Studies in Immuno-oncology Research

We have developed and optimized a 9-color assay and validated its reproducibility across two imaging platforms — the Mantra Workstation and the Vectra Polaris (now rebranded as the PhenoImager HT). In this poster, we demonstrate a translational workflow which offers an optimal balance between multiplexing and sample throughput to facilitate research and support translational studies …

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A Novel Platform for Highly Multiplexed, Single-Cell Imaging of Cell Suspensions

This poster, a collaboration between Akoya and researchers at Stanford University, was a preliminary study into the compatibility of the CODEX® system (now rebranded as PhenoCycler) with cell suspensions. The initial results indicate that the PhenoCycler system can be used as a faster, simpler, and more affordable alternative for cellular biomarker analysis.