Resource: Poster

A Novel H&E-like Staining Method Compatible with Multiplexed IF on the Same Tissue Section for Integrated Translational Workflows

This poster demonstrates a new fluorescent morphology staining method for creating a simulated H&E view while facilitating the integration of mIF analysis methods into digital pathology workflows by giving pathologists familiar, conventional views of mIF-stained tissue sections. It also enables the assessment of tissue quality prior to antigen retrieval treatment and the H&E-based annotation of …

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CODEX® Ultra-high Plex Immunofluorescence Imaging for Neuroscience

This experiment demonstrates the successful implementation of CODEX® to image formalin-fixed paraffin embedded (FFPE) human brain slices. The tissue was stained with a 22 antibody panel to identify neural, glial and vasculature specific cell types. Our data confirm that CODEX® is suitable and immediately deploy-able for neuroscience research.

Analysis of FFPE Human Tumor Tissues Using CODEX with Signal Amplification

We recently expanded our CODEX workflow to amplify the fluorescent signal intensities of low expressing biomarkers by combining Tyramide Signal Amplification (TSA) with CODEX, also known as CODEX® Amp. In this poster, we present the analysis of CODEX data for a panel of over 20 biomarkers on human FFPE tissue, including signal amplification of PD-L1, …

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Developing and Validating a High-throughput 9-color Immunofluorescence Assay for Translational Studies in Immuno-oncology Research

We have developed and optimized a 9-color assay and validated its reproducibility across two imaging platforms — the Mantra Workstation and the Vectra Polaris. In this poster, we demonstrate a translational workflow which offers an optimal balance between multiplexing and sample throughput to facilitate research and support translational studies on whole FFPE tissue.