Using a first-of-its-kind, 103-plex spatial phenotyping panel, this poster demonstrates how whole-slide, single-cell spatial phenotyping uncovers distinct spatial signatures reflecting intratumor heterogeneity and aids in revealing mechanisms of clinical response and therapeutic resistance.
This poster demonstrates a novel protein chemistry that integrates the PhenoCycler and PhenoImager chemistries to simplify panel development.
This poster demonstrates the utility of Akoya’s PD 1 /PD L 1 panel kit in studying the cellular diversity of various cancers while retaining spatial context.
This poster demonstrates how the PhenoCycler platform was used to identify distinct immune phenotypes present in both liver tumor tissues and high-fat-diet livers to better understand how immune dysregulation can predispose an aged and fatty liver to hepatocellular carcinoma.
In this study, authors analyzed tissue using an 46-plex antibody panel with the PhenoCycler-Fusion system and derived targeted 6-plex panels to run on the PhenoImager HT platform aimed at T cell profiling, immune exhaustion, and immuno modulatory targets. Both analyses revealed similar spatial signatures across cohorts.
This study demonstrates the utility of multispectral imaging and spatial analysis of multiple biomarkers in a single tissue sample for characterizing the complex immune landscape of glioblastoma, with potential implications for enhancing design and implementation of therapeutics.
This poster demonstrates the utility of Akoya’s PD-1 /PD-L1 Melanoma panel kit in studying cellular diversity while retaining spatial context. The panel was used to analyze the tumor microenvironment and specifically assess immune phenotypes within melanoma samples from various melanoma tissues.
This poster demonstrates a new fluorescent morphology staining method for creating a simulated H&E view while facilitating the integration of mIF analysis methods into digital pathology workflows by giving pathologists familiar, conventional views of mIF-stained tissue sections. It also enables the assessment of tissue quality prior to antigen retrieval treatment and the H&E-based annotation of …
This experiment demonstrates the successful implementation of CODEX (now rebranded as PhenoCycler) to image formalin-fixed paraffin embedded (FFPE) human brain slices. The tissue was stained with a 22 antibody panel to identify neural, glial and vasculature specific cell types. Our data confirms that the PhenoCycler instrument is suitable and immediately deploy-able for neuroscience research.
This poster, a collaboration between Akoya and researchers at Edinger Institute of Neurology, analyzes the dynamic cell interactions occurring within the malignant brain tumor microenvironment using MOTiF™ in order to understand the biology behind tumor progression.