This poster demonstrates a new fluorescent morphology staining method for creating a simulated H&E view while facilitating the integration of mIF analysis methods into digital pathology workflows by giving pathologists familiar, conventional views of mIF-stained tissue sections. It also enables the assessment of tissue quality prior to antigen retrieval treatment and the H&E-based annotation of …
This experiment demonstrates the successful implementation of CODEX® to image formalin-fixed paraffin embedded (FFPE) human brain slices. The tissue was stained with a 22 antibody panel to identify neural, glial and vasculature specific cell types. Our data confirm that CODEX® is suitable and immediately deploy-able for neuroscience research.
This poster, a collaboration between Akoya and researchers at Edinger Institute of Neurology, analyzes the dynamic cell interactions occurring within the malignant brain tumor microenvironment using MOTiF™ in order to understand the biology behind tumor progression.
This poster demonstrates the development of a 36-marker breast cancer-specific CODEX panel, which was designed to identify different subpopulations of cells in FFPE breast cancer tissue.
We recently expanded our CODEX workflow to amplify the fluorescent signal intensities of low expressing biomarkers by combining Tyramide Signal Amplification (TSA) with CODEX, also known as CODEX® Amp. In this poster, we present the analysis of CODEX data for a panel of over 20 biomarkers on human FFPE tissue, including signal amplification of PD-L1, …
We have developed and optimized a 9-color assay and validated its reproducibility across two imaging platforms — the Mantra Workstation and the Vectra Polaris. In this poster, we demonstrate a translational workflow which offers an optimal balance between multiplexing and sample throughput to facilitate research and support translational studies on whole FFPE tissue.
In this poster, 28-plex analysis of human FFPE tissues was performed with CODEX® to understand spatial interactions in the tumor microenvironment. The CODEX® workflow was adjusted to incorporate TSA-mediated dyes for the amplification of low-expressing markers, including FOXP3, PD-L1, and PD-1.
This poster, a collaboration between Akoya and researchers at Stanford University, was a preliminary study into the compatibility of the CODEX® system with cell suspensions. The initial results indicate that CODEX® can be used as a faster, simpler, and more affordable alternative for cellular biomarker analysis.
In this poster we showcase examples of profiling FFPE and fresh frozen tumor samples with highly multiplexed CODEX panels that include relevant tumor and immune markers.
In this poster we describe the analysis of FFPE samples (human tonsil, melanoma, breast camcer tissues) using the CODEX platform. CODEX antibody staining is also shown in tissue biopsy cores from tissue microarrays (TMAs) as proof of concept.