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Characterizing immune evasion in FFPE tissue sections – a new method for measuring cellular interactions via multiplexed phenotype mapping and spatial point patterns

Authors: Clifford Hoyt, Chichung Wang, Kristin Roman, Kent Johnson, Peter Miller & Elizabeth Mittendorf

Online: https://link.springer.com/article/10.1186/2051-1426-2-S3-P259

Issue: Journal for ImmunoTherapy of Cancer volume 2, Article number: P259 (2014)



Full realization of the potential of immunotherapy will require biomarkers that capture immuno-tumor interactions. This will most effectively be achieved by phenotyping cells in-situ in intact tissue sections. Such methods will potentially predict response to drugs and monitor efficacy and onset of resistance. Existing methods are limited. Here we report a novel integrated approach, utilizing highly multiplexed immunofluorescence labeling, imaging-based analysis to capture expression and cellular interactions in tumor and the microenvironment, and statistical analysis. Of particular interest is applicability of this approach to intact FFPE tissue sections, amenable to routine pathology workflow. The capability of this technology to show the range of immune-tumor interactions is demonstrated on a set of breast cancer samples.



  • Perkin Elmer, Inc. USA
  • MD Anderson Cancer Center, University of Texas, Houston, TX, USA.