Resources

Phenoptics Overview & Case Studies

Suggested Content Resources:


Stain

Suggested Content Resources:


Image

Suggested Content Resources:


Analysis

Suggested Content Resources:


Contract Research Services

Suggested Content Resources:


Applications and Technical Notes

Suggested Content Resources:


Posters

DPA Poster Summaries

Background: In today’s digital pathology realm, introduction of immunofluorescence-based immunohistochemistry (IHC) has allowed for labeling of different targets of interest, as well as greater flexibility, speed, and quality of data collection. However, conventional fluorescence IHC is currently limited to studying 3-4 markers of interest and is often negatively affected by tissue autofluorescence. Our multispectral imaging (MSI) Phenoptics™ platform overcomes these confounds by not only expanding upon the number of markers of interest that can be studied, but through spectral unmixing is able to remove tissue autofluorescence. Until recently, MSI has been limited to analyzing select fields of view and often has long acquisition times.

  • Poster Title: Whole-Slide Multispectral Imaging: Workflows and Applications
    Synopsis: In this poster, we discuss the advancements to our Phenoptics™ technology, wherein we demonstrate a novel, high-throughput method using our new Opal™ Polaris 6-plex, 7-color fluorophores to acquire a whole-slide multispectral image (1 x 1.5cm tissue) in ~6 minutes on the Vectra Polaris. Annotated regions for analysis were selected in Phenochart using the upgraded viewer that allowed for a live unmixing preview, including autofluorescence removal. Further analysis was conducted using inForm and Phenoptr to identify cell phenotypes, locations, and spatial density in order to measure heterogeneity and immune response in the tumor microvienvironment.
  • Poster Title: Overcoming Limitations of Conventional Fluorescence Slide Scanning with Multispectral Approaches
    Synopsis: In this poster, we validate our novel, high-throughput whole slide MSI acquisition workflow by comparing it to conventional whole slide fluorescence imaging and field-based MSI (all optimized for Opal™ fluorophores) and measuring autofluorescence removal and crosstalk, known limiting factors in obtaining usable imagery and quantifiable data in immunofluorescence IHC.

SITC Poster Summaries

Background: Development of clinically relevant immuno-oncology (IO) therapies is dependent upon translational biomarker research. Our current Phenoptics™ platform offers a comprehensive workflow consisting of multiplex fluorescence immunohistochemistry staining, multispectral imaging, and tissue analysis, in which multiple markers of interest can be stained on one tissue sample. This technology allows for cell-to-cell interactions to be observed, as well as revealing the underlying biology occurring within the tumor microenvironment.