TSA coumarin system

TSA Coumarin System

Tyramide Signal Amplification is a technology that improves sensitivity by up to 100-fold sensitivity while allowing reduced consumption of primary antibodies or probes.
TSA Fluorescence Kits use horseradish peroxidase (HRP) to catalyze covalent deposition of fluorophores directly adjacent to the immobilized enzyme. The labeling reaction is quick (less than 10 minutes) and deposited labels can then be viewed directly using standard or confocal microscopy. TSA Fluorescein may also be used in combination with anti-fluorescein enzyme conjugates and appropriate chromogenic substrates for brightfield microscopy.

Catalog Number
Name | Link to Shop Online
NEL703001KT TSA Coumarin System 100-300 slides

Product Details

TSA® coumarin detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra coumarin in the localized area of the probe or antibody.

These systems are designed for direct fluorescence detection in ISH or IHC procedures.

  • Signal amplification for IHC, ISH and related applications
  • Direct fluorescence detection
  • Up to 1000-fold higher sensitivity
  • Conserve precious antibodies or probes
  • High resolution result
  • Adds 2 steps to existing protocol for rapid results

The use of TSA reagents results in a significant increase in sensitivity over standard detection methods, while maintaining specificity and resolution. Moreover, TSA reagents allow drastically reduced consumption of primary antibody or probe.


Shipping Condition Blue Ice
Sample Human
Product Category TSA Kits
Type TSA Fluorescence Kit
Detection Method Mantra and Vectra Quantitative Pathology Imaging Instruments


TSA Quick Start Manual
Protocol at a glance for optimizing TSA for IHC/ICC/IF


Opal Assay Development Guide
User Manual
Comprehensive user manual for Opal staining.


TSA Fluorescence Manual
Data Sheet
Step by step protocols for use.