TSA biotin kits are complete tyramide signal amplification kits that include labeled tyramide, amplification diluent, streptavidin HRP, blocking reagent and comprehensive instruction manuals. Complete TSA Plus Biotin Systems are tyramide signal amplification kits that provide 10 to 20 times the sensitivity enhancement of regular TSA systems. TSA Biotin Reagent Packs provide flexibility to develop custom protocols.
TSA® biotin reagent pack, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. These reagents amplify by depositing extra biotin in the localized area of the probe or antibody.
TSA Biotin Kits use horseradish peroxidase (HRP) to catalyze covalent deposition of biotin labels directly adjacent to the immobilized enzyme. The labeling reaction is quick (less than 10 minutes) and deposited labels can be detected with streptavidin conjugates for imaging in brightfield or fluorescence microscopy.
TSA Biotin kits contain:
|50-150 slides||1X Amplification Diluent (15 mL)
Biotin Tyramide (dry, dissolve in 300 μL DMSO)
Streptavidin-HRP (150 μL)
Blocking Reagent (3 g)