TSA biotin kit

TSA Biotin

TSA biotin kits are complete tyramide signal amplification kits that include labeled tyramide, amplification diluent, streptavidin HRP, blocking reagent and comprehensive instruction manuals. Complete TSA Plus Biotin Systems are tyramide signal amplification kits that provide 10 to 20 times the sensitivity enhancement of regular TSA systems. TSA Biotin Reagent Packs provide flexibility to develop custom protocols.

Catalog Number
Name | Link to Shop Online
NEL700A001KT TSA Biotin System 50-150 slides 50-150 slides
NEL749A001KT TSA Plus Biotin 50-150 slides 50-150 slides
SAT700001EA TSA Biotin Reagent Pack 200-600 Slides 200-600 Slides

Product Details

TSA® biotin reagent pack, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. These reagents amplify by depositing extra biotin in the localized area of the probe or antibody.

TSA Biotin Kits use horseradish peroxidase (HRP) to catalyze covalent deposition of biotin labels directly adjacent to the immobilized enzyme. The labeling reaction is quick (less than 10 minutes) and deposited labels can be detected with streptavidin conjugates for imaging in brightfield or fluorescence microscopy.


TSA Biotin kits contain:

50-150 slides 1X Amplification Diluent (15 mL)
Biotin Tyramide (dry, dissolve in 300 μL DMSO)
Streptavidin-HRP (150 μL)
Blocking Reagent (3 g)


Shipping Condition Blue Ice
Sample Human
Product Category TSA
Type TSA Hapten Kits
Detection Method Mantra and Vectra Quantitative Pathology Imaging Instruments


TSA Quick Start Manual
Protocol at a glance for optimizing TSA for IHC/ICC/IF


Opal Assay Development Guide
User Manual
Comprehensive user manual for Opal staining.


TSA and TSA Plus Biotin Manuals
Data Sheet
Step by step protocols

Download TSA Biotin Data Sheet
Download TSA Plus Biotin Data Sheet

Material Safety Data Sheets

Catalog Number
NEL700A001KT TSA Biotin System 50-150 slides Download
NEL749A001KT TSA Plus Biotin 50-150 slides Download