Opal Reagents Quick Start

Fast start to using Opal Technology with custom markers

Opal Reagents Quick Start

Fast start to using Opal Technology with custom markers

Opal Quick Start Tool

Unlock the power of spatial biology with any tissue with your choice of biomarkers with Opal.

Getting started with the Opal technology has never been easier. The guidance on this page is based on published data from top researchers that have already leveraged the technology, providing valuable insight for how to get started.

The guidance and support initiated by your answers will help us to better support your successful implementation.

Choose the tissue type you’re working with

Brain Tissue

In a study on chronic traumatic encephalopathy (CTE), Cherry et. al. used biochemical and histological approaches to characterize tau isoform signatures in post-mortem human brain tissue across a range of CTE stages. Using Phenoptics reagents, instruments, and software they visualized 4R Tau, 3R Tau, AT8, MAP2, GFAP, and Iba1 on FFPE tissue.

Used in this study

Cardiac Tissue

Peyster et. al. found that quantitative multiplexed immune-phenotyping of heart transplant biopsies provides novel diagnostic and prognostic information about allograft health and could be used to predict cardiac allograft rejection and severity. Phenoptics reagents, instruments, and software were used to visualize and quantify CD3, CD8, CD68, FoxP3 and PD-L1 on human FFPE tissue.

Used in this study

Kidney Tissue

In a study on kidney fibrosis, Lovisa et. al. observed that tubular epithelial cells (TECs) acquire a partial epithelial–to–mesenchymal transition program which induces cell cycle arrest and lower expression of several transporters in TECs. Using Phenoptics instruments and reagents, they visualized YFP, αSMA, PH3, β-Catenin, and Ki67 in mouse FFPE tissue.

Used in this study

Liver Tissue

Saldarriaga et. al. analyzed five antibodies commonly observed on macrophage populations to characterize intrahepatic macrophages in patients with chronic liver diseases. They found multispectral imaging ideal for analyzing human liver tissues, as it allows spectral unmixing of fluorophore signals, subtraction of auto-fluorescence, and preservation of hepatic architecture. Using Phenoptics reagents, instruments, and software, they visualized CD68, CD14, CD16, CD163, and MAC387 in human FFPE tissue.

Used in this study

Other Tissues

Working with another tissue type? Use the form below to tell us about your project – our team can provide guidance for your tissue of interest.

Discuss your project needs with us

Fill out the form below to tell us about the samples you’re working with and your biomarkers of interest. One of our technical specialists will get in touch to provide expert support for your project.

  • 1DESCRIBE YOUR SAMPLE
  • 2SELECT MARKERS
  • This field is required.
    Please select which markers from these publications you would like to use. You may also type any other markers of interest separated by a comma or press enter.
    CD4CD5CD6
  • 3FILL IN CONTACT INFORMATION

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