Opal 7 Immunology Discovery Kit
The Opal™ 7 Immunology Discovery Kit enables phenotyping of T cells, B cells and macrophages, with 3 open channels for user-selected targets.
Catalog Number |
Name | Link to Shop Online |
Size |
| OP7DS2001KT | Opal 7 Immunology Discovery Kit | 50 slides |
Product Details
Opal™ is a practical workflow for the simultaneous detection of up to six tissue biomarkers plus nuclear counterstain within a single image, similar to standard immunohistochemistry (IHC).
The Opal™ 7 Immunology Discovery Kit includes all the critical workflow reagents to enable phenotyping of T cells, B cells and macrophages, with 3 open channels for user-selected targets, using 6-plex fluorescent immunohistochemistry:
- Antibodies to CD4, CD8 & CD68
- Antibody diluent
- 6-plex fluorescent IHC detection reagents
- Spectral DAPI
- Antigen retrieval buffers
To develop an IHC custom panel for investigation of the immune response, the user pairs this kit with antibodies to 3 additional targets of interest. This kit was configured and validated for multispectral imaging on the Vectra and Mantra Systems. Contains enough reagents for 6-plex immunostain of 50 slides.
- Optimized panel for assessment of immune cells in intact FFPE tumor tissue
- Shortest path to results
- Part of the Phenoptics™ workflow for immunophenotyping of solid tumors
- Flexibility for detection of your targets of interest in multiplex panels
- Faster time-to-results
Specifications
| Shipping Condition | Blue Ice |
| Sample | Human |
| Product Category | Opal Kits |
| Type | Opal Detection Kit |
| Detection Method | Mantra and Vectra Quantitative Pathology Imaging Instruments |
Workflow
- Bake at 60 C for one hour
- Xylene wash (3x ten minutes)
- Rehydrate with EtOH gradient into di H2O
- 10% NBF for 20 minutes
- Di H2O wash
- AR6 or AR9 MWT
- Cool to RT for at least 15 minutes
- Di H2O rinse, TBST rinse
- PAP pen barrier
- Incubate tissue for 10 minutes in blocking solution at RT
- Remove blocker and add primary antibody
- Rinse with TBST, then wash 3 times for two minutes each in TBST
- Incubation 10 minutes at RT
- Rinse with TBST and wash 3 times for 2 minutes each
- Opal fluorophore incubation for ten minutes at RT
- Rinse with TBST and wash 3 times for 2 minutes each
- Rinse slides with AR6 or AR9
- Place slides in microwave-safe jar, perform MWT
- Cool to RT for at least 15 minutes
- Use AR6 for MWT prior to DAPI for removal of any unbound fluor
- Rinse slides in microwave-safe jar, perform MWT
- Incubate DAPI for 5 minutes at RT
- Wash with di H2O and TBST two minutes each
- Apply monunting medium for fluorescene microscopy for coverslip
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