Opal Antibody Diluent / Block

This ready-to-use antibody diluent and blocking buffer formulation has been optimized specifically for Opal™ and TSA® Plus immunohistochemistry protocols.

Product Details

This 2-in-1 antibody diluent and blocking buffer acts to minimize non-specific interaction of antibodies that can be a source of background
This formulation does not contain azide and should be compatible with all peroxidase based detection methods. Size: 50 mL.

  • Ready to use formulation
  • Convenient dropper bottle
  • Azide-free, does not affect HRP

Specifications

Shipping Condition Blue Ice
Sample Human and Mouse
Product Category Opal Kits
Type Opal Ancillary Reagents
Detection Method Mantra and Vectra Quantitative Pathology Imaging Instruments

Workflow

Bake and Deparaffinize Slides
  • Bake at 60 C for one hour
  • Xylene wash (3x ten minutes)
  • Rehydrate with EtOH gradient into di H2O
Slide Fixation
  • 10% NBF for 20 minutes
  • Di H2O wash
Antigen Retrieval
  • AR6 or AR9 MWT
  • Cool to RT for at least 15 minutes
Blocking
  • Di H2O rinse, TBST rinse
  • PAP pen barrier
  • Incubate tissue for 10 minutes in blocking solution at RT
Primary Antibody
  • Remove blocker and add primary antibody
  • Rinse with TBST, then wash 3 times for two minutes each in TBST
Secondary Antibody
  • Incubation 10 minutes at RT
  • Rinse with TBST and wash 3 times for 2 minutes each
Opal Reagent
  • Opal fluorophore incubation for ten minutes at RT
  • Rinse with TBST and wash 3 times for 2 minutes each
MWT
  • Rinse slides with AR6 or AR9
  • Place slides in microwave-safe jar, perform MWT
  • Cool to RT for at least 15 minutes
Spectral DAPI
  • Use AR6 for MWT prior to DAPI for removal of any unbound fluor
  • Rinse slides in di H2O and then TBST
  • Incubate DAPI for 5 minutes at RT
  • Wash with di H2O and TBST two minutes each
Mount
  • Apply monunting medium for fluorescene microscopy for coverslip

Documents

Phenoptics Portflolio
Brochure

Download

Opal Assay Development Guide
User Manual
Comprehensive user manual for Opal staining.

Download