Mantra 2™ Quantitative Pathology Workstation

Akoya is proud to present the next generation of the Mantra family; the Mantra 2™ Quantitative Pathology Workstation. Building on the momentum from the launch of Phenoptics 2.0, this workstation can now be optionally configured to detect up to 8 markers. This is an ideal system for labs just getting started with multiplexing, and also for high-throughput labs looking to streamline their assay development workflow alongside Vectra® Polaris ™.

Product Details

The Mantra 2™ Quantitative Pathology Workstation incorporates multispectral imaging technology, image acquisition and analysis with the inForm® software, and can be used with a variety of stains including Akoya’s Opal™ reagent kits.

Traditional methods like flow cytometry and next-generation sequencing allow you to phenotype and quantify immune cells in homogenized samples, but you lose critical spatial information and context. Meanwhile, standard pathological analyses can deliver morphology data but do not allow the analysis of complex phenotypes.

With multispectral imaging, you can phenotype and quantify immune cells from images of FFPE tissue sections, maintaining tissue architecture, cellular spatial relationships and morphological context. This capability available on the Mantra 2™ provides spatial information, allowing for a better understanding of the role and types of immune cells within both the tumor and the tumor microenvironment so that new cancer immunotherapy treatments may be identified and researched.

Key Benefits

  • Visualizes, analyzes, quantifies and phenotypes immune cells in situ in solid tissues
  • Ideal for assay development, freeing up higher throughput systems, such as the Vectra Polaris
  • Intuitive workflow that uses LED light source to maximize efficiency for the research pathologist
  • Compact system that fits on your benchtop
  • Eye-piece for easy visual checking of work as you go
  • Optional 9 color multispectral fluorescence upgrade path
  • Liquid crystal tunable filter (LCTF) technology
  • Supports Akoya’s Phenoptics™ workflow solution for cancer immunology research


Imaging Modes Brightfield and fluorescence, multispectral and color imaging
Microscope Customized research-grade microscope with:

  • 8-position motorized epi-fluorescence filter turret
  • 7-position motorized objective nosepiece
  • Active management of intensity per-objective in brightfield
  • Integrated ergonomic controls on microscope base
Camera Type Multispectral 12-bit camera using cooled scientific grade CCD
Camera Spectral Range 440-760nm, 780nm for Opal Fluorophores
Objectives (Apochromatic) 4x 0.16 NA; 10x 0.40 NA; 20x 0.75 NA; (optional) 40x 0.75
Pixel Resolution 4x = 2.5um; 10x = 1.0um; 20x = 0.5um; (optional) 40x = 0.25um
Filter Cubes
  • DAPI, FITC, Cy3, Texas Red, Cy5
  • Optionally configured with Opal 480, Opal 780, or Quad-band for visualization
Fluorescent Illumination 6 channel LED; >20,000 hour bulb life
Brightfield Illumination High-output, color-balanced white LED
Product Brand Name Mantra 2™
Automation Compatible no
Detection Method Fluorescence, Brightfield
Light Source LED

Support Documents

Phenoptics Portflolio


From Biomarker Discovery to Assay Validation and Beyond
Technical Note


Quantitative Pathology Imaging and Analysis
inForm Product Note