TSA Coumarin System
Tyramide Signal Amplification is a technology that improves sensitivity by up to 100-fold sensitivity while allowing reduced consumption of primary antibodies or probes. TSA Fluorescence Kits use horseradish peroxidase (HRP) to catalyze covalent deposition of fluorophores directly adjacent to the immobilized enzyme. The labeling reaction is quick (less than 10 minutes) and deposited labels can then be viewed directly using standard or confocal microscopy. TSA Fluorescein may also be used in combination with anti-fluorescein enzyme conjugates and appropriate chromogenic substrates for brightfield microscopy.
TSA® coumarin detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra coumarin in the localized area of the probe or antibody.
These systems are designed for direct fluorescence detection in ISH or IHC procedures.
- Signal amplification for IHC, ISH and related applications
- Direct fluorescence detection
- Up to 1000-fold higher sensitivity
- Conserve precious antibodies or probes
- High resolution result
- Adds 2 steps to existing protocol for rapid results
The use of TSA reagents results in a significant increase in sensitivity over standard detection methods, while maintaining specificity and resolution. Moreover, TSA reagents allow drastically reduced consumption of primary antibody or probe.