The Manual Opal 7-Color IHC Kit contains:

• 6 reactive fluorophores (Opal 520, Opal 540, Opal 570, Opal 620, Opal 650 and Opal 690)
• Spectral DAPI
• 1 X Plus Amplification Diluent
• Blocking/Ab Diluent
• Opal Polymer HRP Ms + Rb
• AR6 Buffer

The Automated Opal 7-Color IHC Kit contains:

• 6 reactive fluorophores (Opal 520, Opal 540, Opal 570, Opal 620, Opal 650 and Opal 690)
• Spectral DAPI
• 1 X Plus Automation Amplification Diluent
• Blocking/Ab Diluent
• Opal Polymer HRP Ms + Rb

Users should source primary antibodies and other workflow reagents separately. The Opal 7-Color Manual IHC Kit was optimized for use on the Vectra^® and Mantra™ systems. Kits contain enough reagents to stain 50-slides at the recommended 1:100 or 1:150 dilution ratio.

Opal provides researchers with the tools to interrogate multiple pathways while retaining context provided by tissue images. This approach provides information not available from alternative techniques like flow cytometry or analysis of single markers in serial sections.

Opal will enable you to:

• Measure up to six tissue biomarkers at once
• Use the best primary antibodies together in multiplex panel, with no species-based crosstalk
• Retain spatial cellular context that is lost in flow cytometry
• Confirm single cell co-expression for many biomarkers in one tissue section
• Get more information while conserving precious tissue.