Introducing PhenoCode Discovery Panels
Simplify your spatial biology journey through the ever-expanding biomarker landscape with PhenoCode Discovery Panels.
PhenoCycler - Reagents & Antibodie
The PhenoCyler® solution is a complete suite of antibodies and reagents optimized and validated for use with the PhenoCycler system. We also offer an ever-expanding menu of antibodies that includes essential immune markers. Users have the flexibility to create panels from ready-to-use PhenoCycler inventoried antibodies that have been validated for use across multiple tissue types including, but not limited to, mouse fresh-frozen, human fresh-frozen, and human formalin-fixed paraffin-embedded (FFPE). Custom conjugation kits are also available for labeling antibodies with PhenoCycler barcodes. PhenoCylcer barcodes allow for easy conjugation of antibodies, while the PhenoCycler Reporters are Fluorophore-conjugated oligonucleotides that visualize the antibodies.
Kits & Reagents
The PhenoCycler System utilizes reagents and buffers that have been optimized to be used in combination with PhenoCycler antibodies, barcodes and reporters. For each imaging cycle, three spectrally distinct PhenoCycler Reporters, are applied to the stained tissue to visualize the corresponding PhenoCycler antibody. A series of washes remove the PhenoCycler Reporters, followed by the application of the next set of reporter.
Kits & Reagents
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PhenoCycler Antibodies
PhenoCycler antibodies are provided individually and can be tailored to design panels for the simultaneous analysis of 100+ biomarkers. Akoya provides fully validated PhenoCycler antibodies and a list of supplemental antibodies that have undergone varying levels of testing.
Community Antibodies
- PhenoCycler user referenced 3rd party antibodies
- End-user custom conjugation required
- Minimal level of support by Akoya
Inventoried Antibodies
- Manufactured and purified by an Akoya partner
- End-user custom conjugation required
- Limited support by Akoya
Screened Antibodies
- Designed, conjugated and manufactured by Akoya
- Fully validated for use on PhenoCycler system
- Supported by Akoya
Discovery Panel Modules
PhenoCycler Antibodies For Human FFPE Tissue
PhenoCycler antibodies have been tested in multiple types of human FFPE tissue and are conjugated to a distinct PhenoCycler Barcode (BX000). Twenty-five (25) test scan be performed with each kit.
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