Introducing PhenoCode Discovery Panels
Simplify your spatial biology journey through the ever-expanding biomarker landscape with PhenoCode Discovery Panels.
PhenoCycler - Reagents & Antibodies
The PhenoCyler® solution is a complete suite of antibodies and reagents optimized and validated for use with the PhenoCycler system. We also offer an ever-expanding menu of antibodies that includes essential immune markers. Users have the flexibility to create panels from ready-to-use PhenoCycler inventoried antibodies that have been validated for use across multiple tissue types including, but not limited to, mouse fresh-frozen, human fresh-frozen, and human formalin-fixed paraffin-embedded (FFPE). Custom conjugation kits are also available for labeling antibodies with PhenoCycler barcodes. PhenoCylcer barcodes allow for easy conjugation of antibodies, while the PhenoCycler Reporters are Fluorophore-conjugated oligonucleotides that visualize the antibodies.
Kits & Reagents
Buffers and reagents for tissue staining, custom conjugations and performing PhenoCycler experiments
PhenoCycler Antibodies
A comprehensive menu of pre-conjugated, pre-screened, and pathologist reviewed antibodies
PhenoCycler Barcodes
Modified oligonucleotides for easy conjugation of custom PhenoCycler antibodies
PhenoCycler Reporters
Fluorophore-conjugated oligonucleotides for visualization of PhenoCycler antibodies
Kits & Reagents
The PhenoCycler System requires specially formulated reagents and buffers for tissue staining with PhenoCycler antibodies and running iterative imaging cycles. For each imaging cycle, three PhenoCycler reporters, each with a spectrally distinct dye, are applied to the stained tissue to visualize the corresponding PhenoCycler antibody. The PhenoCycler instrument applies gentle wash steps to remove the PhenoCycler reporters without any chemical modifications, followed by applying the next set of Reporters to visualize the next set of PhenoCycler antibodies.
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PhenoCycler Antibodies
PhenoCycler antibodies are provided individually and can be tailored to design panels for the simultaneous analysis of100+ biomarkers. Akoya provides fully validated PhenoCycler antibodies and a list of supplemental antibodies that have undergone varying levels of testing.
PhenoCycler Inventoried Antibodies
PhenoCycler inventoried antibodies are supplied by Akoya and have passed the PhenoCycler antibody validation process and manufacturing quality control. Our inventoried antibodies are also fully supported by our field support team.
Discovery Panel Modules
PhenoCode Discovery Panel modules are sets of PhenoCycler antibodies and reporters that have been validated to work together on control tissue and cancer tissue. These panel modules can be supplemented with other PhenoCycler antibodies.
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PhenoCycler Antibodies For Human FFPE Tissue
PhenoCycler antibodies have been tested in multiple types of human FFPE tissue and are conjugated to a distinct PhenoCycler Barcode (BX000). Twenty-five (25) test scan be performed with each kit.
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