We’ve rebranded some of our products, learn more ›
CODEX is now PhenoCycler
Phenoptics is now Phenolmager
PhenoCycler – Rapid, Deep Spatial Phenotyping
Characterizing the complexities of the tissue microenvironment is critical in understanding the molecular and cellular mechanisms to unravel insights into disease development, differentiation, and response. The PhenoCycler™-Fusion allows spatial phenotyping of millions of cells at unprecedented scale and speed. Mapping millions of cells and their interactions in a single intact tissue section facilitates the discovery of rare cell types, cellular neighborhoods, and spatial functional states. Push the frontiers of spatial biology with your team using the PhenoCycler solutions (formerly CODEX®).
Benefits
Fast
Sample-To-Data Within
24 Hours
24 Hours
Unbiased
Whole-Slide Imaging at Single-Cell Resolution
Ultrahigh-plex
Image 100+ Biomarkers
Flexible
Compatible With Any Sample Type
Case Studies
Rare Cells and Cellular Neighborhoods: Novel Discoveries from Spatial Phenotyping
Explore how whole-tissue, single-cell imaging enabled the study of a rare cell type and the impact of cellular neighborhoods on tumor progression
Pushing the Frontiers of CAR T-cell Therapy:
A Spatial Analysis of Solid Tumors
See how to use PhenoCycler, a single-cell spatial phenotyping solution, to characterize the microenvironment of various pediatric tumors and to understand the potential interactions that chimeric antigen receptor (CAR) T cells may elicit.
Spatial Multiomics: Enriching Single-Cell Sequencing Data with Spatial Phenotyping
In this multi-part webinar series, our expert speakers review analytical frameworks and algorithms to integrate imaging-based single-cell spatial phenotyping data with complementary transcriptomic and genomic datasets.
Imaging Gallery
Lung
Human Lung – FFPE tissue stained with 39-plex panel
Breast
Adenocarcinoma – FFPE tissue stained with 36-plex panel
Skin
Human skin tissue of psoriasis rash – FFPE tissue stained with 23-plex panel
Technology
Watch this video to learn how the PhenoCycler instrument uses the reveal, image, remove workflow to spatially phenotype cells while retaining tissue context and maintaining sample integrity. Learn how this simple but practical instrument gives you unmatched flexibility to phenotype every single cell across the whole slide giving access to unbiased spatial phenotyping.
Workflow
Stain
Image
Analyze
Stain
Select antibodies and reagents optimized and validated for detection of 100+ biomarkers across the whole slide using the PhenoCycler instrument.
Image
The PhenoCycler instrument enables iterative cycling and imaging by seamlessly integrating with the Fusion imaging platform or any microscope of your choice giving you the power to image on your own terms.
Analyze
Phenotype cells and perform advanced spatial analysis using Akoya Software suite or a variety of open-source applications.
Related Publications
Coordinated cellular neighborhoods orchestrate antitumoral immunity at the colorectal cancer invasive front
2020 Sep 03
This study provides a framework for interrogating how complex biological processes, such as antitumoral immunity, occur through concerted actions of cells and spatial domains.
An N-Cadherin 2 expressing epithelial cell subpopulation predicts response to surgery, chemotherapy, and immunotherapy in bladder cancer
2021 Aug 12
This study combines single-cell sequencing data and CODEX to identify epithelial subpopulations with therapeutic response prediction ability in bladder cancer.
Technological advances in cancer immunity: from immunogenomics to single-cell analysis and artificial intelligence
2021 Aug 20
Summary of conventional and state-of-the-art technologies in the field of immunogenomics, single-cell and artificial intelligence, and present prospects for future research.
