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PhenoImager – Rapid, accurate spatial phenotyping
Unraveling the complex and dynamic tumor microenvironment requires multiplex immunofluorescence imaging to assess a variety of tumor and immunologic biomarkers. Spatial Phenotypic Signatures are a new class of highly predictive biomarker which measure the interactions and cellular densities of tumor and immune cells that compose the tumor microenvironment.
Multiplex imaging utilizing novel biomarkers can reveal the spatial relationships between tumor and immune cells, leading to the discovery of spatial phenotypic signatures while preserving the architectural features of the tumor microenvironment.
Our PhenoImager® Instruments, powered by patented Multispectral Imaging (MSI) Technology, are the fastest quantitative digital pathology imaging platforms enabling rapid and accurate spatial phenotyping at scale.
Pave the path for spatial phenotypic signatures with your team using the PhenoImager.
Benefits
Fast
Fastest Spatial Phenotyping Imagers in the world
Accurate
Patented MSI Technology Enables Quantitative Spatial Phenotyping
Integrated
Integrated Multiplex Immunofluorescence Imaging Solution Optimized from Sample to Result
Efficient
Get More Data by Multiplexing While Saving Precious Samples
Case Studies
First Multi-Institutional Analytical Demonstration of a Spatial Biology Workflow
The MITRE study, published in the Journal for ImmunoTherapy of Cancer, established the high reproducibility and robustness of Akoya Biosciences’ PhenoImager mutliplex immnuofluorescence platform for spatial phenotyping in clinical research.
Top immuno-oncology and pathology experts from five institutions collaborated with Akoya to conduct the MITRE study, including Johns Hopkins University School of Medicine, Yale University School of Medicine, Earle A. Chiles Research Institute, The University of Texas MD Anderson Cancer Center, and Bristol Myers Squibb.
Top immuno-oncology and pathology experts from five institutions collaborated with Akoya to conduct the MITRE study, including Johns Hopkins University School of Medicine, Yale University School of Medicine, Earle A. Chiles Research Institute, The University of Texas MD Anderson Cancer Center, and Bristol Myers Squibb.
Spatial Biomarkers in Immuno-Oncology: Recent Developments and Future Directions
In this four-part webinar series, researchers discuss pioneering new strategies representing significant milestones that advance Spatial Biology into the realm of patient phenotyping and improving immunotherapy outcomes in the future. Hear from our expert panel on recent developments that establishes the vital role of Spatial Biomarkers in setting the future directions in achieving Precision Immuno-Oncology.
Contact sports and Chronic Traumatic Encephalopathy (CTE)
Learn how researchers used multiplex immunofluorescence to study various Tau isoforms and identify early pathological changes in CTE
Imaging Gallery
Human Lung
FFPE tissue stained with a 6-plex panel
Human Brain
FFPE tissue stained with a 5-plex panel
Human Heart
FFPE tissue stained with a 6-plex panel
Human Kidney
FFPE tissue stained with a 6-plex panel
Human Melanoma
FFPE tissue stained with a 6-plex panel
From our experience in applying multiplexed imaging for immuno-oncology research, the PhenoImager/Opal platform is an effective high-throughput solution to profile the immune landscape of various microcompartments in tumor sections. The Opal staining kits are versatile and easy to use with autostainers, and the Polaris image acquisition is fast and consistent. Speed and capacity are important for research in the pharmaceutical industry.
Department of Translational Sciences,
Rakuten Medical Inc.
Having the PhenoImager HT (formerly Vectra Polaris) in our lab has transformed our ability to analyze and understand the tumor immune microenvironment… PhD students with little to no experience of IHC have quickly mastered the ability to perform multiplex IHC … Before obtaining this technology, we were unable to carry out equivalent immunohistochemistry analyses... The increased information and thus understanding of the tumor microenvironments this technology is revealing to us will greatly help inform us in our development of more effective combination immunotherapies.
Previous
Next
Technology
Watch this video to learn about how the PhenoImager instruments use a patented Multispectral Imaging (MSI) Technology and spectral unmixing to isolate autofluorescence and biomarkers of interest regardless of their intensity or any spectral overlap into separate channels, providing accurate and quantitative data for spatial phenotyping applications.
Workflow
Stain
Image
Analyze
Stain
Select from predesigned fully validated panels or pair your markers of interest with our Opal kits to simultaneous detect up to 8 biomarkers within a single tissue section
Image
Available in two options, the PhenoImager Fusion instrument is ideal for laboratories with standard throughput needs while the PhenoImager HT instrument provides the perfect solution for high throughput multiplex immunofluorescence imaging applications
Analyze
Remove autofluorescence and seamlessly integrate images into a variety of whole slide analysis software for advanced spatial phenotyping analysis
Related Publications
Spatially organized multicellular immune hubs in human colorectal cancer
2021 Aug 26
Researchers at The Broad Institute apply multiplex immunofluorescence imaging to show how immune cell hubs are organized in structured cell neighborhoods within human colorectal cancers.
Immune infiltrate dynamics and response to chemotherapy in breast cancer
2021 Aug 12
West German Study Group researchers utilize multiplexed immunohistochemistry to predict response to neoadjuvant chemotherapy in triple-negative breast cancer (TNBC).
Role of tertiary lymphoid structures (TLSs) in response to immunotherapy
2021 Aug 20
University of Bordeaux team applies multiplex immunofluorescence imaging to identify mature TLSs within several solid tumors and show their association with better outcomes for cancer patients treated with immunotherapy.
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