Highly Multiplexed Single-Cell Spatial Analysis of FFPE Tumor Tissues Using CODEX (now rebranded as PhenoCycler)

In this poster, 28-plex analysis of human FFPE tissues was performed with PhenoCycler to understand spatial interactions in the tumor microenvironment. The PhenoCycler workflow was adjusted to incorporate TSA-mediated dyes for the amplification of low-expressing markers, including FOXP3, PD-L1, and PD-1.