A Novel H&E-like Staining Method Compatible with Multiplexed IF on the Same Tissue Section for Integrated Translational Workflows

This poster demonstrates a new fluorescent morphology staining method for creating a simulated H&E view while facilitating the integration of mIF analysis methods into digital pathology workflows by giving pathologists familiar, conventional views of mIF-stained tissue sections. It also enables the assessment of tissue quality prior to antigen retrieval treatment and the H&E-based annotation of mIF imagery and supports eventual translation of mIF methods into clinical standards of care.