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This technical note describes the validation process for commercial CODEX® antibodies. We show how the CODEX® antibodies perform similarly to other immunofluorescence (IF) and immunohistochemistry (IHC) grade dye-conjugated antibodies.
In this poster, 28-plex analysis of human FFPE tissues was performed with CODEX® to understand spatial interactions in the tumor microenvironment. The CODEX® workflow was adjusted to incorporate TSA-mediated dyes for the amplification of low-expressing markers, including FOXP3, PD-L1, and PD-1.
This poster, a collaboration between Akoya and researchers at Stanford University, was a preliminary study into the compatibility of the CODEX® system with cell suspensions. The initial results indicate that CODEX® can be used as a faster, simpler, and more affordable alternative for cellular biomarker analysis.
In this poster we showcase examples of profiling FFPE and fresh frozen tumor samples with highly multiplexed CODEX panels that include relevant tumor and immune markers.
In this poster we describe the analysis of FFPE samples (human tonsil, melanoma, breast camcer tissues) using
the CODEX platform. CODEX antibody staining is also shown in tissue biopsy cores from tissue microarrays (TMAs)
as proof of concept.
In this poster we describe the complete CODEX Solution that encompasses reagents, the CODEX instrument and the software suite. We also demonstrate some data on fresh frozen samples and how the software can enable clustering and cellular phenotyping of the tissue microenvironment.
In this poster we show the chemistry behind CODEX technology and demonstrate the compatibility of the system with different tissue types.