Overview of CODEX® Portfolio
- Technical Note: CODEX® Commercial Antibody Validation
Background: This technical note describes the validation process for commercial CODEX® antibodies. The CODEX technology allows tissues to be stained with an entire antibody panel in a single step, thus decreasing experiment time and preserving sample integrity. In this technical note we show how the CODEX antibodies perform similarly to other immunofluorescence (IF) and immunohistochemistry (IHC) grade dye-conjugated antibodies. We also describe the quantitative and qualitative validation process to ensure the right specificity is achieved while optimizing sensitivity. And finally, we also demonstrate an assay for measuring potential steric hindrance from CODEX antibody panels and show that there are minimal effects of this from the demonstrated example.
Background: In this poster we showcase examples of profiling FFPE and fresh frozen tumor samples with highly multiplexed CODEX panels that include relevant tumor and immune markers. Tissue types analyzed are renal cell carcinoma (FFPE), breast cancer (FFPE) and metastatic breast cancer lymph node (Fresh Frozen). Using the metastatic breast cancer lymph node tissue as an example, we've also demonstrated how complex the tissue architecture and detailed cellular phenotypes can be uncovered using a highly multiplexed panel of markers.
- Poster Title: Spatially-resolved deep biomarker profiling of single cells in FFPE tissue samples through CODEX®
Background: In this poster we describe the analysis of FFPE samples using the CODEX platform. Spatially resolved, deep biomarker profiling of tissue specimens is crucial for investigating the architecture and cell diversity in complex matrices, such as the tumor microenvironment (TME). CODEX assays can be performed on a variety of tissue sections, ranging from FFPE to fresh frozen samples. CODEX assays are also non-destructive: tissues can be cleared and stored after one or more CODEX runs. In the poster we show representative images of CODEX multicycles on FFPE human tonsil, melanoma and breast cancer tissues. CODEX antibody staining is also shown in tissue biopsy cores from tissue microarrays (TMAs) as proof of concept.
- Poster Title: CODEX®: A novel platform for spatially-resolved deep biomarker profiling of single cells in tissue sample
Background: In this poster we describe the complete CODEX Solution that encompasses reagents, the CODEX instrument and the software suite. We also demonstrate some data on fresh frozen samples and how the software can enable clustering and cellular phenotyping of the tissue microenvironment.
- Poster Title: Multiparametric Proteomic Profiling Via Imaging Dozens of Biomarkers Simultaneously
Background: In this poster we show the chemistry behind CODEX technology – single step staining with the multiplexed antibody panel followed by iterative cycles of labeling, imaging and removing Reporters using the fully automated CODEX fluidics unit, until all biomarkers of interest are imaged. We demonstrate the compatibility of the system with different tissue types – Spleen, Lymph Note and Tonsil and FFPE melanoma and the ability of the platform to multiplex 40+ markers in a single panel. And finally, this poster also shows examples of how to analyze highly multiplexed tissue images to uncover complex cellular interactions and phenotypes.