PhenoCycler-Fusion Assays
Scalable and Flexible Deep Spatial Phenotyping
We’ve rebranded some of our products, learn more ›
CODEX® is now PhenoCycler,
Phenoptics™ is now Phenolmager.
Unlock Deep Spatial Discoveries with PhenoCycler-Fusion 2.0 Assays
No matter the scale of your experiment—be it a 20-plex study, a 40-plex exploration, or a deep 100-plex spatial phenotyping assay—PhenoCycler®-Fusion 2.0 Assays can seamlessly adapt to the needs of your spatial discoveries. Powered by proprietary barcoded antibody technology and leveraging an extensive database of over 350 antibodies, PhenoCycler®-Fusion 2.0 Assays make deep spatial phenotyping easier than ever.
Choose from ready-to-use PhenoCode™ Discovery Panels for time-efficient studies or design your own panels to suit your specific research needs. With the PhenoCyler-Fusion 2.0 system and PhenoCycler®-Fusion 2.0 Assays, you can embark on a spatial journey where scalability meets unparalleled flexibility, transforming the pace of your spatial biology research.
What Sets PhenoCycler-Fusion Assays Apart?
Scalable Plexing
Unmatched Content
Unparalleled Flexibility
How does Barcoding Technology Work?
The multiplexing capability of the PhenoCycler-Fusion 2.0 system is underpinned by the fundamental principles of barcoded antibody technology. In this framework:
- Each PhenoCycler antibody is intricately linked to a unique oligonucleotide sequence, known as the PhenoCycler Barcode.
- Each PhenoCycler Reporter is a fluorescent dye molecule tagged with a short, complementary oligonucleotide sequence designed specifically for the associated PhenoCycler antibody.
- When the barcoded antibody and the reporter with the complementary oligo are incubated they undergo hybridization. This results in the generation of a fluorescent signal that can be effectively captured for each biomarker of interest.
Faster Discoveries with Flexible Panel Solutions
Want to Simplify Your Research with Ready-to-use Panels?
Designed for the ever-expanding biomarker universe, PhenoCode Discovery Panels simplify end-to-end workflows for spatial discoveries on the PhenoCycler-Fusion 2.0 platform.
- Ultrahigh-plex protein panels
- Curated panel design based on cancer hallmarks
- Modular design for scalability
- 3X faster assay development and optimization
Want to Design Your Own Panels?
Explore Akoya's extensive antibody database, a dynamic and ever-growing resource designed to build your own panels.
Ready to Order PhenoCycler-Fusion Reagents?
PhenoCode Discovery Panels and Antibodies
PhenoCode Discovery Panels
Antibodies
PhenoCycler Antibodies For Human FFPE Tissue
PhenoCycler antibodies have been tested in multiple types of human FFPE tissue and are conjugated to a distinct PhenoCycler Barcode (BX000). Twenty-five (25) test scan be performed with each kit.
PhenoCycler Inventoried Antibodies for Human Fresh Frozen Tissue
PhenoCycler antibodies for human fresh frozen tissue have been tested using tonsil tissue. These PhenoCycler antibodies are conjugated to a distinct PhenoCycler barcode (BX000). Each kit contains one tube of PhenoCycler antibody and one tube of the corresponding dye-labeled reporter (RX000), sufficient volume for 25 PhenoCycler runs (or tests) at the standard staining conditions. Recommended dyes for human fresh frozen tissue are Atto 550, Cy5 or Alexa Fluor™ 647, and Alexa Fluor™ 488.
PhenoCycler Inventoried Antibodies for Mouse Fresh Frozen Tissue
PhenoCycler antibodies for mouse fresh frozen tissue have been tested using spleen tissue and are conjugated to a distinct PhenoCycler barcode (BX000). Twenty-five (25) tests can be performed with each kit.