The CODEX® Solution

Highly multiplexed immunofluorescence has arrived on your benchtop

The CODEX® Solution

Highly multiplexed immunofluorescence has arrived on your benchtop

CODEX Assay Reagents and Antibodies

The CODEX Assay overcomes challenges of traditional multiplexed immunofluorescence, by targeting subsets of the antibody panel through iterative imaging cycles, thus revealing 40+ biomarkers in a single tissue section.

We offer a complete solution that includes antibodies and reagents that have been optimized and validated for the CODEX System. Validated and inventoried antibodies are available for different tissue types: Mouse Fresh Frozen, Human Fresh Frozen and Human FFPE. Users have the flexibility to create panels comprised of commercially available, Akoya-validated antibodies or clones labeled with CODEX Barcodes using Akoya’s custom conjugation kit.

Buffers and reagents for tissue staining, custom conjugations and performing CODEX experiments

CODEX conjugated antibodies for building antibody panels

Modified oligonucleotides for easy conjugation of custom CODEX antibodies

Fluorophore conjugated oligonucleotides for visualization of CODEX antibodies

 

Kits & Reagents

The CODEX Assay requires specially formulated reagents and buffers for tissue staining with CODEX Antibodies and running iterative imaging cycles. For each imaging cycle, three CODEX Reporters, each with a spectrally-distinct dye, are applied to the stained tissue to visualize the corresponding CODEX Antibody. The CODEX instrument applies gentle wash steps to remove the CODEX Reporters without any chemical modifications, followed by applying the next set of Reporters to visualize the next set of CODEX Antibodies.

Catalog #
Name
size
7000001 10X CODEX Buffer 10 reactions
7000002 CODEX Assay Reagent 10 reactions
7000003 Nuclear Stain 10 reactions
7000008 CODEX Staining Kit 10 reactions
7000009 CODEX Conjugation Kit 10 reactions
7000010 CODEX Gaskets v2 10 gaskets
232107 CODEX Storage Buffer 120 ml
7000005 Coverslips 138
7000006 96 well plates 10
7000007 96 well plate seals 10

 

CODEX Antibodies

CODEX Antibodies are provided individually and can be mixed and matched to design panels for the simultaneous analysis of up to 40+ biomarkers.

Akoya provides fully validated CODEX Antibodies and a list of supplemental antibodies that have undergone varying levels of testing.

 

CODEX® Inventoried Antibodies

CODEX Inventoried Antibodies are supplied by Akoya and have passed the full CODEX Antibody Validation Process and manufacturing quality control. Our inventoried antibodies are also fully supported by our field support team.

CODEX® Antibodies For Human FFPE Tissue

CODEX Antibodies have been tested in multiple types of human FFPE tissue. These CODEX Antibodies are conjugated to a distinct CODEX Barcode (BX000). Each kit contains one tube of CODEX Antibody and one tube of the corresponding dye-labeled Reporter (RX000), sufficient volume for 25 CODEX runs (or tests) at the standard staining conditions. Recommended dyes for FFPE tissue are Atto 550, Cy5, and Alexa Fluor™ 750.

Catalog #
Name
Size
4450036 b-Catenin1-BX020 (12F7)—Atto 550-RX020 25 tests
4450030 CD3e-BX045(EP449E)—Cy5-RX045 25 tests
4350018 CD4-BX003 (EPR6855)—Cy5-RX003 25 tests
4250012 CD8-BX026 (C8/144B)—Atto 550-RX026 25 tests
4350020 CD11c-BX024 (118/A5)—Cy5-RX024 25 tests
4150018 CD20-BX007 (L26)—Alexa Fluor™ 488-RX007 25 tests
4450018 CD20-BX007 (L26)—Alexa Fluor™ 750-RX007 25 tests
4450027 CD21-BX032 (EP3093)—Atto 550-RX032 25 tests
4150017 CD31-BX001 (EP3095)—Alexa Fluor™ 488-RX001 25 tests
4450017 CD31-BX001 (EP3095)—Alexa Fluor™ 750-RX001 25 tests
4250002 CD44-BX005 (IM7)—Atto 550-RX005 25 tests
4350002 CD45-BX021 (2D1)—Cy5-RX021 25 tests
4250023 CD45RO-BX017 (UCHL1)—Atto 550-RX017 25 tests
4350019 CD68-BX015 (KP1)—Cy5-RX015 25 tests
4350001 CD107a-BX006 (H4A3)—Cy5-RX006 25 tests
4250021 E-cadherin-BX014 (4A2C7)—Atto 550-RX014 25 tests
4350021 Histone H3 Phospho (Ser28)-BX030 (HTA28)—Cy5-RX030 25 tests
4450029 HLA-DR-BX033(EPR3692)—Cy5-RX033 25 tests
4450031 Keratin 14-BX002(Poly19053)—Atto 550-RX002 25 tests
4250019 Ki67-BX047 (B56)—Atto 550-RX047 25 tests
4450032 LIF-BX006(M1506B09)—Cy5-RX006 25 tests
4450034 Mac2/Galectin-3-BX035(M3/38)—Atto 550-RX035 25 tests
4150020 Pan-Cytokeratin-BX019 (AE-1/AE-3)—Alexa Fluor™ 488-RX019 25 tests
4450020 Pan-Cytokeratin-BX019 (AE-1/AE-3)—Alexa Fluor™ 750-RX019 25 tests
4250004 Podoplanin-BX023 (NC-08)—Atto 550-RX023 25 tests
4450033 TFAM-BX029(18G102B2E11)—Atto 550-RX029 25 tests
CODEX® Antibodies For Human Fresh Frozen Tissue

CODEX Antibodies for human fresh frozen tissue have been tested using tonsil tissue. These CODEX Antibodies are conjugated to a distinct CODEX Barcode (BX000). Each kit contains one tube of CODEX Antibody and one tube of the corresponding dye-labeled Reporter (RX000), sufficient volume for 25 CODEX runs (or tests) at the standard staining conditions. Recommended dyes for human fresh frozen tissue are Atto 550, Cy5, and Alexa Fluor™ 488.

Catalog#
Name
Size
4250005 CD2-BX002 (RPA-2.10)—Atto 550-RX002 25 tests
4350008 CD3-BX015 (UCHT1)—Cy5-RX015 25 tests
4350010 CD4-BX021 (SK3)—Cy5-RX021 25 tests
4150004 CD8-BX004 (SK1)—Alexa Fluor™ 488-RX004 25 tests
4450004 CD8-BX004 (SK1)—Alexa Fluor™ 750-RX004 25 tests
4350012 CD11c-BX027 (S-HCL-3)—Cy5-RX027 25 tests
4350003 CD19-BX003 (HIB19)—Cy5-RX003 25 tests
4150009 CD21-BX013 (Bu32)—Alexa Fluor™ 488-RX013 25 tests
4450009 CD21-BX013 (Bu32)—Alexa Fluor™ 750-RX013 25 tests
4250009 CD31-BX032 (WM59)—Atto 550-RX032 25 tests
4250020 CD34-BX035 (561)—Atto 550-RX035 25 tests
4150007 CD38-BX007 (HB-7)—Alexa Fluor™ 488-RX007 25 tests
4450007 CD38-BX007 (HB-7)—Alexa Fluor™ 750-RX007 25 tests
4150003 CD45-BX001 (HI30)—Alexa Fluor™ 488-RX001 25 tests
4450003 CD45-BX001 (HI30)—Alexa Fluor™ 750-RX001 25 tests
4250023 CD45RO-BX017 (UCHL1)—Atto 550-RX017 25 tests
4350007 CD49f-BX033 (GoH3)—Cy5-RX033 25 tests
4250022 CD69-BX041 (FN50)—Atto 550-RX041 25 tests
4150021 CD90-BX022 (5E10)—Alexa Fluor™ 488-RX022 25 tests
4450021 CD90-BX022 (5E10)—Alexa Fluor™ 750-RX022 25 tests
4250008 CD104-BX005 (58XB4)—Atto 550-RX005 25 tests
4150008 CD138-BX010 (MI15)—Alexa Fluor™ 488-RX010 25 tests
4450008 CD138-BX010 (MI15)—Alexa Fluor™ 750-RX010 25 tests
4250013 CD278-BX017 (C398.4A)—Atto 550-RX017 25 tests
4250010 CD279-BX014 (EH12.2H7)—Atto 550-RX014 25 tests
4250006 HLA-DR-BX026 (L243)—Atto 550-RX026 25 tests
4250019 Ki67-BX047 (B56)—Atto 550-RX047 25 tests
4150020 Pan-Cytokeratin-BX019 (AE-1/AE-3)—Alexa Fluor™ 488-RX019 25 tests
4450020 Pan-Cytokeratin-BX019 (AE-1/AE-3)—Alexa Fluor™ 750-RX019 25 tests
4250004 Podoplanin-BX023 (NC-08)—Atto 550-RX023 25 tests
CODEX® Antibodies For Mouse Fresh Frozen Tissue

CODEX Antibodies for mouse fresh frozen tissue have been tested using spleen tissue. These CODEX Antibodies are conjugated to a distinct CODEX Barcode (BX000). Each kit contains one tube of CODEX Antibody and one tube of the corresponding dye-labeled Reporter (RX000), sufficient volume for 25 CODEX runs (or tests) at the standard staining conditions. Recommended dyes for mouse fresh frozen tissue are Atto 550, Cy5, and Alexa Fluor™ 488.

Catalog#
Name
Size
4350014 CD3-BX021 (17A2)—Cy5-RX021 25 tests
4250016 CD4-BX026 (RM4-5)—Atto 550-RX026 25 tests
4250007 CD5-BX017 (53-7.3)—Atto 550-RX017 25 tests
4250017 CD8a-BX029 (53-6.7)—Atto 550-RX029 25 tests
4150015 CD11b-BX025 (M1/70)—Alexa Fluor™ 488-RX025 25 tests
4450015 CD11b-BX025 (M1/70)—Alexa Fluor™ 750-RX025 25 tests
4350013 CD11c-BX030 (N418)—Cy5-RX030 25 tests
4250014 CD19-BX020 (6D5)—Atto 550-RX020 25 tests
4250015 CD21/35-BX023 (7E9)—Atto 550-RX023 25 tests
4150014 CD24-BX022 (M1/69)—Alexa Fluor™ 488-RX022 25 tests
4450014 CD24-BX022 (M1/69)—Alexa Fluor™ 750-RX022 25 tests
4250001 CD31-BX002 (MEC13.3)—Atto 550-RX002 25 tests
4150013 CD38-BX019 (90)—Alexa Fluor™ 488-RX019 25 tests
4450013 CD38-BX019 (90)—Alexa Fluor™ 750-RX019 25 tests
4250002 CD44-BX005 (IM7)—Atto 550-RX005 25 tests
4150002 CD45-BX007 (30-F11)—Alexa Fluor™ 488-RX007 25 tests
4450002 CD45-BX007 (30-F11)—Alexa Fluor™ 750-RX007 25 tests
4150006 CD45R/B220-BX010 (Ra3-6B2)—Alexa Fluor™ 488-RX010 25 tests
4450006 CD45R/B220-BX010 (Ra3-6B2)—Alexa Fluor™ 750-RX010 25 tests
4350007 CD49f-BX033 (GoH3)—Cy5-RX033 25 tests
4350016 CD71-BX027 (RI7217)—Cy5-RX027 25 tests
4450001 CD90.2-BX001 (30-H12)—Alexa Fluor™ 750-RX001 25 tests
4150001 CD90.2-BX001 (30-H12)—Alexa Fluor™ 488-RX001 25 tests
4350005 CD169-BX015 (3D6.112)—Cy5-RX015 25 tests
4150012 IgD-BX016 (11-26c.2a)—Alexa Fluor™ 488-RX016 25 tests
4450012 IgD-BX016 (11-26c.2a)—Alexa Fluor™ 750-RX016 25 tests
4150011 IgM-BX013 (RMM-1)—Alexa Fluor™ 488-RX013 25 tests
4450011 IgM-BX013 (RMM-1)—Alexa Fluor™ 750-RX013 25 tests
4250019 Ki67-BX047 (B56)—Atto 550-RX047 25 tests
4350015 Ly6g-BX024 (1A8)—Cy5-RX024 25 tests
4250003 MHC II-BX014 (M5/114.15.2)—Atto 550-RX014 25 tests
4350006 TCRb-BX003 (H57-597 )—Cy5-RX003 25 tests
4150005 Ter119-BX004 (TER-119)—Alexa Fluor™ 488-RX004 25 tests
4450005 Ter119-BX004 (TER-119)—Alexa Fluor™ 750-RX004 25 tests

 

CODEX® Screened Antibodies

CODEX Screened Antibodies are not inventoried or supplied by Akoya but include a list of clones that have been purified and successfully conjugated to CODEX Barcodes and have shown positive staining signal in secondary lymphoid tissue. The clones are commercially available from other antibody vendors but require conjugation by the end user. CODEX Barcodes and the associated conjugation protocol are available through Akoya.

 

CODEX® Community Antibodies

The CODEX user base is growing and we are encouraging collaboration across our customer sites to share their list of CODEX-tested clones and protocols. These CODEX Community antibodies, while demonstrated to be compatible with the CODEX instrument, will not be sold through Akoya. Conjugation is required by the end user. CODEX Barcodes and the associated conjugation protocol are available through Akoya. We are continuing to expand the list.

 

CODEX® Antibody Custom Panel Design

Akoya’s CODEX system provides users the flexibility to create panels comprised of Akoya-validated antibodies or clones labeled with CODEX Barcodes using the custom conjugation kit. Users can use the CODEX Conjugation Kit and individual CODEX Barcodes to robustly prepare clones from third-party antibody suppliers.

 

CODEX Barcodes

CODEX Barcodes are used with the CODEX Conjugation kit for custom antibody panel design. Each kit contains one tube of the lyophilized Barcode for use in a single conjugation reaction, and two tubes of Reporters for use in optimizing CODEX antibody titration and CODEX runs. For each barcode, there is sufficient volume for 50 µg antibody conjugation, which provides enough material for 10 to 50 stains depending on yield and titer.

CODEX Barcodes with Alexa Fluor™ 488 Reporters
Catalog#
Name
Size
5450013 BX001—Alexa Fluor™ 488-RX001 1 conjugation reaction
5450014 BX004—Alexa Fluor™ 488-RX004 1 conjugation reaction
5450015 BX007—Alexa Fluor™ 488-RX007 1 conjugation reaction
5450016 BX010—Alexa Fluor™ 488-RX010 1 conjugation reaction
5450017 BX013—Alexa Fluor™ 488-RX013 1 conjugation reaction
5150001 BX016—Alexa Fluor™ 488-RX016 1 conjugation reaction
5150002 BX019—Alexa Fluor™ 488-RX019 1 conjugation reaction
5150003 BX022—Alexa Fluor™ 488-RX022 1 conjugation reaction
5150004 BX025—Alexa Fluor™ 488-RX025 1 conjugation reaction
5150005 BX028—Alexa Fluor™ 488-RX028 1 conjugation reaction
5150006 BX031—Alexa Fluor™ 488-RX031 1 conjugation reaction
5150007 BX034—Alexa Fluor™ 488-RX034 1 conjugation reaction
5150008 BX037—Alexa Fluor™ 488-RX037 1 conjugation reaction
5150009 BX040—Alexa Fluor™ 488-RX040 1 conjugation reaction
5150010 BX043—Alexa Fluor™ 488-RX043 1 conjugation reaction
5150011 BX046—Alexa Fluor™ 488-RX046 1 conjugation reaction
5150012 BX049—Alexa Fluor™ 488-RX049 1 conjugation reaction
CODEX Barcodes with Alexa Fluor™ 750 Reporters
Catalog#
Name
Size
5450018 BX001—Alexa Fluor™ 750-RX001 1 conjugation reaction
5450019 BX004—Alexa Fluor™ 750-RX004 1 conjugation reaction
5450020 BX007—Alexa Fluor™ 750-RX007 1 conjugation reaction
5450021 BX010—Alexa Fluor™ 750-RX010 1 conjugation reaction
5450022 BX013—Alexa Fluor™ 750-RX013 1 conjugation reaction
5450001 BX016—Alexa Fluor™ 750-RX016 1 conjugation reaction
5450002 BX019—Alexa Fluor™ 750-RX019 1 conjugation reaction
5450003 BX022—Alexa Fluor™ 750-RX022 1 conjugation reaction
5450004 BX025—Alexa Fluor™ 750-RX025 1 conjugation reaction
5450005 BX028—Alexa Fluor™ 750-RX028 1 conjugation reaction
5450006 BX031—Alexa Fluor™ 750-RX031 1 conjugation reaction
5450007 BX034—Alexa Fluor™ 750-RX034 1 conjugation reaction
5450008 BX037—Alexa Fluor™ 750-RX037 1 conjugation reaction
5450009 BX040—Alexa Fluor™ 750-RX040 1 conjugation reaction
5450010 BX043—Alexa Fluor™ 750-RX043 1 conjugation reaction
5450011 BX046—Alexa Fluor™ 750-RX046 1 conjugation reaction
5450012 BX049—Alexa Fluor™ 750-RX049 1 conjugation reaction
CODEX Barcodes with Atto 550 Reporters
Catalog#
Name
Size
5450023 BX002—Atto 550-RX002 1 conjugation reaction
5450024 BX005—Atto 550-RX005 1 conjugation reaction
5450025 BX014—Atto 550-RX014 1 conjugation reaction
5250001 BX017—Atto 550-RX017 1 conjugation reaction
5250002 BX020—Atto 550-RX020 1 conjugation reaction
5250003 BX023—Atto 550-RX023 1 conjugation reaction
5250004 BX026—Atto 550-RX026 1 conjugation reaction
5250005 BX029—Atto 550-RX029 1 conjugation reaction
5250006 BX032—Atto 550-RX032 1 conjugation reaction
5250007 BX035—Atto 550-RX035 1 conjugation reaction
5250008 BX041—Atto 550-RX041 1 conjugation reaction
5250009 BX047—Atto 550-RX047 1 conjugation reaction
CODEX Barcodes with Cy5 Reporters
Catalog#
Name
Size
5450026 BX003—Cy5-RX003 1 conjugation reaction
5450027 BX006—Cy5-RX006 1 conjugation reaction
5350001 BX015—Cy5-RX015 1 conjugation reaction
5350002 BX021—Cy5-RX021 1 conjugation reaction
5350003 BX024—Cy5-RX024 1 conjugation reaction
5350004 BX027—Cy5-RX027 1 conjugation reaction
5350005 BX030—Cy5-RX030 1 conjugation reaction
5350006 BX033—Cy5-RX033 1 conjugation reaction
5350007 BX036—Cy5-RX036 1 conjugation reaction
5350008 BX042—Cy5-RX042 1 conjugation reaction
5350009 BX045—Cy5-RX045 1 conjugation reaction

 

CODEX Reporters

For each imaging cycle, three CODEX Reporters, each with a spectrally-distinct dye, are applied to the stained tissue to assay the corresponding Antibody Barcode. The tissue is imaged, revealing the fluorescence signal levels of the first three targets plus a nuclear stain.

CODEX Reporters are included in the CODEX Antibodies and CODEX Barcodes Kits and are also available to order individually.

CODEX Alexa Fluor™ 488 Reporters
Catalog#
Name
Size
6150001 Alexa Fluor™ 488-RX001 25 tests
6150002 Alexa Fluor™ 488-RX004 25 tests
6150003 Alexa Fluor™ 488-RX007 25 tests
6150004 Alexa Fluor™ 488-RX010 25 tests
6150005 Alexa Fluor™ 488-RX013 25 tests
6150006 Alexa Fluor™ 488-RX016 25 tests
6150007 Alexa Fluor™ 488-RX019 25 tests
6150008 Alexa Fluor™ 488-RX022 25 tests
6150009 Alexa Fluor™ 488-RX025 25 tests
6150010 Alexa Fluor™ 488-RX028 25 tests
6150011 Alexa Fluor™ 488-RX031 25 tests
6150012 Alexa Fluor™ 488-RX034 25 tests
6150013 Alexa Fluor™ 488-RX037 25 tests
6150014 Alexa Fluor™ 488-RX040 25 tests
6150015 Alexa Fluor™ 488-RX043 25 tests
6150016 Alexa Fluor™ 488-RX046 25 tests
6150017 Alexa Fluor™ 488-RX049 25 tests
CODEX Alexa Fluor™ 750 Reporters
Catalog#
Name
Size
6450001 Alexa Fluor™ 750-RX001 25 tests
6450002 Alexa Fluor™ 750-RX004 25 tests
6450003 Alexa Fluor™ 750-RX007 25 tests
6450004 Alexa Fluor™ 750-RX010 25 tests
6450005 Alexa Fluor™ 750-RX013 25 tests
6450006 Alexa Fluor™ 750-RX016 25 tests
6450007 Alexa Fluor™ 750-RX019 25 tests
6450008 Alexa Fluor™ 750-RX022 25 tests
6450009 Alexa Fluor™ 750-RX025 25 tests
6450010 Alexa Fluor™ 750-RX028 25 tests
6450011 Alexa Fluor™ 750-RX031 25 tests
6450012 Alexa Fluor™ 750-RX034 25 tests
6450013 Alexa Fluor™ 750-RX037 25 tests
6450014 Alexa Fluor™ 750-RX040 25 tests
6450015 Alexa Fluor™ 750-RX043 25 tests
6450016 Alexa Fluor™ 750-RX046 25 tests
6450017 Alexa Fluor™ 750-RX049 25 tests
CODEX Atto 550 Reporters
Catalog#
Name
Size
6250001 Atto 550-RX002 25 tests
6250002 Atto 550-RX005 25 tests
6250003 Atto 550-RX014 25 tests
6250004 Atto 550-RX017 25 tests
6250005 Atto 550-RX020 25 tests
6250006 Atto 550-RX023 25 tests
6250007 Atto 550-RX026 25 tests
6250008 Atto 550-RX029 25 tests
6250009 Atto 550-RX032 25 tests
6250010 Atto 550-RX035 25 tests
6250011 Atto 550-RX041 25 tests
6250012 Atto 550-RX047 25 tests
CODEX Cy5 Reporters
Catalog#
Name
Size
6350001 Cy5-RX003 25 tests
6350002 Cy5-RX006 25 tests
6350003 Cy5-RX015 25 tests
6350004 Cy5-RX021 25 tests
6350005 Cy5-RX024 25 tests
6350006 Cy5-RX027 25 tests
6350007 Cy5-RX030 25 tests
6350008 Cy5-RX033 25 tests
6350009 Cy5-RX036 25 tests
6350010 Cy5-RX042 25 tests
6350011 Cy5-RX045 25 tests