The CODEX® Solution

Ultrahigh multiplexed imaging has arrived on your benchtop

The CODEX® Solution

Ultrahigh multiplexed imaging has arrived on your benchtop

A Comprehensive Solution for Spatially Resolved, Multiplexed Immunofluorescence

Characterizing the complexities of the tissue microenvironment is a critical element in understanding the molecular and cellular mechanisms driving disease as well as therapeutic responses.

The roles of spatial relationships between infiltrating immune cells and the remodeling of the cellular matrix are widely recognized as key components that define tissue heterogeneity. The CODEX System enables researchers to image 40+ protein markers in tissue samples at single cell resolution. Highly multiplexed imaging enables the study of different cell phenotypes and how they interact in situ to impact disease pathology and progression.

CODEX Vornoi diagram

CODEX Technology

The CODEX (CO-Detection by indEXing) technology uses an antibody conjugated to proprietary Akoya Barcodes, comprised of a unique oligonucleotide sequence. The CODEX Assay targets specific barcodes with a dye-labeled Reporter for highly specific detection.

The tissue sample is stained with the CODEX antibody panel in a single step and the Reporters bind to the complementary Barcodes through iterative cycles of imaging and removal. The cycles are fully automated by the CODEX fluidics instrument.

Flexible

Design custom panels for fresh frozen or FFPE tissues

Sample Friendly

Samples can be re-imaged for region of interest (ROI) analysis

Scalable

Image 40+ markers in a single tissue sample at single-cell resolution

Comprehensive

Includes reagents and software analysis suite

CODEX Multiplexed Imaging Examples

The CODEX Solution is compatible with many tissue types and formats.

Breast cancer tissue stained with a 36-plex CODEX panel

Human Breast FFPE tissue stained with a 36-plex panel

  • E-cadherin
  • KRT19
  • KRT17
  • b-actin
  • Ki67
  • CD31
  • DAPI

Lung cancer tissue stained with a 28-plex CODEX panel

Lung Cancer FFPE tissue stained with a 28-plex panel

  • Pan-Cytokeratin
  • CD8
  • Ki67
  • PD-L1
  • PD-1
  • CD68

Human Skin Fresh Frozen tissue stained with a 26-plex panel

  • Pan-Cytokeratin
  • CD138
  • CD49f
  • CD45
  • CD34
  • Podoplanin
  • CD31

Human PBMCs fixed and stained with a 26-plex panel

  • Ki67
  • CD45
  • CD2
  • CD31
  • CD63
  • CD45
  • CD7

Renal Cell Carcinoma FFPE tissue stained with a 15-plex panel

  • Ki67
  • CD8
  • CD4
  • Podoplanin
  • SMA
  • CD20
  • CD31

Experimental Workflow

01

Stain

Prepare and stain tissue with the CODEX Antibody Panel in a single step thus reducing turnaround time and preserving sample integrity.

02

Image

Image 40+ markers in your tissue with the fully automated CODEX instrument and a companion microscope. Users have the ability to go down to single-cell resolution with a 20X objective and subcellular resolution with a 40X objective.

03

Analyze

Process, analyze and visualize your images with the comprehensive CODEX Software Suite. Users can access visualization tools like gating and clustering for cellular phenotyping. Users can also export files for use in third-party software.

Andras A Heczey, M.D.

Assistant Professor, Pediatrics-Oncology, Dan L Duncan Comprehensive Cancer Center

Baylor College of Medicine

Postinfusion biopsies are extremely precious… With CODEX our aim is to get the most data from small samples of tissues to understand as much of the tumor microenvironment as possible.

Andras A Heczey, M.D.

Assistant Professor, Pediatrics-Oncology, Dan L Duncan Comprehensive Cancer Center

Baylor College of Medicine

Postinfusion biopsies are extremely precious… [With CODEX] our aim is to get the most data from small samples of tissues to understand as much of the tumor microenvironment as possible.

Garry Nolan, PhD

Rachford and Carlota A. Harris Professor, Department of Microbiology and Immunology

Stanford University, Co-Founder, Akoya Biosciences

The commercial launch of CODEX is a proud milestone for my lab and an exciting opportunity for biomedical sciences. The ability to simultaneously and economically analyze dozens or more protein markers on intact tissues will fundamentally change our understanding of cellular interactions and disease biology.

Garry Nolan, PhD

Rachford and Carlota A. Harris Professor, Department of Microbiology and Immunology

Stanford University, Co-Founder, Akoya Biosciences

The commercial launch of CODEX is a proud milestone for my lab and an exciting opportunity for biomedical sciences. The ability to simultaneously and economically analyze dozens or more protein markers on intact tissues will fundamentally change our understanding of cellular interactions and disease biology.

Case Studies

The Powers & Brissova Research Group at the Vanderbilt Diabetes Center uses CODEX to study the pancreatic islet microenvironment.

The Cell Sciences Imaging Facility (CSIF) at Stanford University expands their core lab services with multiplexed imaging projects on the CODEX System.

Researchers at University of Pennsylvania have developed a method to map single-cell CITE-seq data to CODEX multiplex IHC images for spatial, single-cell multi-omics studies.